Induction of Agarwood in Aquilaria malaccensis Using Nitrogen Fertilizer and  Fusarium solani)

Resti Wahyuni; Triadiati Triadiati; Syamsul Falah

doi:10.18330/jwallacea.2018.vol7iss2pp165-171

Authors

Resti Wahyuni
resti_bio@yahoo.com
Program Pascasarjana Program Studi Biologi Tumbuhan, Fakultas Matematika dan IPA, Institut Pertanian Bogor Jl. Raya Darmaga Kampus IPB Darmaga Bogor 16680, Telp/Faks : +62 2518622833, Indonesia
Triadiati Triadiati Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor Jl. Raya Darmaga, Kampus IPB Darmaga Bogor 16680, Telp/Faks.: +62 2518622833, Indonesia
Syamsul Falah Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor Jl. Raya Darmaga, Kampus IPB Darmaga Bogor 16680, Telp/Faks.: +62 2518625481/+62 2518625708, Indonesia

Vol. 7 No. 2 (2018)

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August 31, 2018

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Aquilaria malaccensis is agarwood producing species in Indonesia. Agarwood compounds are formed as a chemical response of Aquliaria malaccensis tree to various physical damages, phatogen infection, or chemical treatment. Factors influencing agarwood formation are age of the tree, season, geographical location, environment, and treatment period. Agarwood induction may be done in tree or sapling. Agarwood induction in saplings need more effort than in trees. Combination of fungi (Fusarium solani) and nutrient (Nitrogen fertilizer) treatment may be one way to induce agarwood in A. malaccensis saplings. This study aims to produce A. malaccensis agarwood (aromatic compounds and colour) by induction of F. solani and nitrogen fertilizer, and analyse the agarwood chemical content. The agarwood chemical content was investigated by Gas Chromatography Mass Spectrometry (GCMS) analysis. Results indicated that agarwood had a different colour for every treatment. The darkest brown and most fragrant agarwood were produced by A. malaccensis treated by a combination of nitrogen fertilizer (4 gr/sapling) and F. solani inoculation. Three chemical compounds were identified i.e. silanediol dimethyl, 4-ethyl benzoic acid and 1,4,7,10,13,16- hexaoxacyclooctadecane with percentages of 25.7, 17.62, and 3.56 respectively. A. malaccensis treated by nitrogen fertilizer and F. solani for 3 months is able to induce aromatic compounds formation, but the colour still dark brown. Biosynthesis of aromatic compounds in agarwood occurs first before changes in the colour of the wood.

Wahyuni, R., Triadiati, T., & Falah, S. (2018). Induction of Agarwood in Aquilaria malaccensis Using Nitrogen Fertilizer and Fusarium solani). Jurnal Penelitian Kehutanan Wallacea, 7(2), 165-171. https://doi.org/10.18330/jwallacea.2018.vol7iss2pp165-171

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Ahuja, I., Kissen, R., & Bones, AM. (2012). Phytoalexins in defense against pathogens. Trends in Plant Science, 12(2),73-90.

Akter, N., & Neelim, AZ. (2008). Agarwood plantation at BRAC tea estate: introduction, environmental factors and financial analysis. http://research.brac.net/reports/Agarwood_Plan tation_BRAC.pdf Accessed 5 August 2016.

Akter, S., Islam, MD., Zulkefeli, M., Khan, SI. (2013). Agarwood production a multidisiplinary field to be explored in Bangladesh. International Journal of Pharmaceutical and Life Science, 2(1),22–32.

Amin, MRM., Bejo, SK., Ismail, WIW., Mashohor, S. (2012). Colour extraction of agarwood images for fuzzy C-means classification. Walailak Journal of Science and Technology, 9(4), 445–459.

Azah, MN., Husni, SS., Mailina, J., Sahrim, L., Majid, JA., & Faridz, ZM. (2013). Classification of agarwood by resin content. Journal of Tropical Forest Science, 25(2), 213–219.

Bryant RJ, McClung AM (2011) Volatile profiles of aromatic and non-aromatic rice cultivars using SPME/GC–MS. Food Chem, 124(2), 501–513.

CITES. (2010, November). Appendix II of convention on international trade in endangered species of wild fauna and flora. Diakses dari https://cites.org/eng/notif/2010/E007A.pdf.

Compton, J., & Ishihara, A. (2004). The use and trade of agarwood in Japan. A TRAFFIC report to the CITES Secretariat (Vol. 6). Retrieved from http://144.76.93.178/sites/default/files/commo n/com/pc/15/X-PC15-06-Inf.pdf.

Faizal, A., Esyanti, RR., Aulianisa, EN., Iriawati., Santoso, E., Turjaman, M. (2016). Formation of agarwood from Aquilaria malaccensis in response to inoculation of local strains of Fusarium solani. Trees. doi:10.1007/s00468-016-1471-9.

Handa, SS., Khanuja, SPS., Longo, G., Rakesh, DD. (2008). Extraction technologies for medicinal and aromatic plants .ICS-UNIDO, Italy.

Jong, PL. (2012). Effects of mechanical wounding and infection patterns of Fusarium solani on gaharu formation in Aquilaria malaccensis Lam.[disertasi]. Malaysia (MY): Universitas Putra Malaysia.

Kakino, M., Tazawa, S., Maruyama, H., Tsuruma, K., Araki, Y., Shimazawa, M., & Hara, H. (2010). Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complementary and Alternative Medicine, 10, 68-75.

Li, W., Cai, CH., Guo, ZK., Wang, H., Zuo, WJ., Dong, WH., Mei, WL., & Dai, HF. (2015). Five new eudesmane type sesquiterpenoids from Chinese agarwood induced by artificial holing. Fitoterapia, 100, 44– 49.

Liu, Y., Chen, H., Yang, Y., Zhang, Z., Wei, J., Meng, H., Chen, W., Feng, J., Gan, B., Chen, X., Gao, Z., Huang, J., Chen, B., Chen, H. (2013). Whole-tree agarwood-inducing technique: an efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees. Molecules, 18(3), 3086–3106.

Mazid, M., Khan, TA., Mohammad, F. (2011). Role of secondary metabolites in defense mechanisms of plants. Biology and Medicine, 3(2), 232–249.

Mohamed, R., Lee, JP., & Kudus, KA. (2014). Fungal inoculation induced agarwood in young Aquilaria malaccensis trees in the nursery. Journal of Forestry Research, 25(1), 201–204.

Ng, LT., Chang, YS., Kadir, AA. (1997). A review on agar (gaharu) producing Aquilaria species. Journal of Tropical Forest Products, 2, 272-285.

Novriyanti, E., Santosa, E. (2011). The role of phenolics in agarwood formation of Aquilaria crassna Pierre ex Lecomte and Aquilaria microcarpa Baill. Indonesia Journal of Forestry Research, 8(2), 101– 113.

Nurbaya., Kuswinanti, T., Rosmana, A., Baharuddin., Millang, S. (2014). Growth rate and identification of Fusarium sp assosiated with Aquillaria sp from Nunukan regency, North Kalimantan. International Journal Current Research and Academic Review, 2(11), 33–40.

Ohyama, T. (2010). Nitrogen Assimilation in Plants. Japan: Niigata University.

Okudera, Y., Ito, M. (2009). Production of agarwood fragrant constituents in Aquilaria calli and cell suspension cultures. Plant Biotechnology, 26(3), 307–315.

Persoon G (2007) Agarwood: the life of a wounded tree. IIAS Newslett, 45, 24–25.

Rahayu,G., Isnaeni, Y., Umboh, MIJ. (1999). Potensi beberapa himofiset dalam induksi gejala pembentukan gubal gaharu. di dalam: Seminar kongres nasional ke XV dan seminar ilmiah perhimpunan fitopatologi Indonesia; Purwokerto, 1999 Sept 16-18; Purwokerto, Indonesia. Purwokerto (ID): Universitas jendral Sudirman. hlm:1-6.

Rahayu,G., Santoso, E., Yunita, L. (2009). Interaksi Acremonium sp. dan metil jasmonat dalam pembentukan gubal gaharu pada Aquilaria microcarpa. di dalam: Proceedings and seminar program international seminar and the 20th national congress of Indonesian phytopathological society; Makasar, 2009 Agu 4-7; Makasar, Indonesia. Makasar (ID): Universitas Hasanudin. hlm 18.

Singh, M., Khan, MM., Naeem, M. (2014). Effect of nitrogen on growth, nutrient assimilation, essential oil content, yield and quality attributes in Zingiber officinale Rosc. Journal of the Saudi Society of Agricultural Sciences, 15(2), 171-178.

Zhang, Z., Wei, J., Han, X., Liang, L., Yang, Y., Meng, H., Xu, Y., Gao, Z. (2014). The sesquiterpene biosynthesis and vessel-occlusion formation in stems of Aquilaria sinensis (Lour.) Gilg trees induced by wounding treatments without variation of microbial communities. International Journal of Molecular Sciences, 15(12), 23589-23603.