AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI BAGIAN RANTING DAN BATANG TUMBUHAN KARAMUNTING (Melastoma malabathricum)
Antioxidant and Antibacterial Activity of The Twigs and Stem Bark of Karamunting Plants (Melastoma malabathricum)
DOI:
https://doi.org/10.24259/perennial.v17i2.14547Keywords:
Antioxidant, antibacterial, DPPH, Melastoma malabathricumAbstract
East Kalimantan is one of the areas that have the potential for various traditional medicinal plants. Melastoma malabathricum (known locally as karamunting, senduduk) is an invasive plant in the family of Melastomataceae with a potential medicinal plant. This study explored the potential of twigs and stem bark of Karamunting (Melastoma malabathricum) for its antioxidant and antibacterial activity. The twigs and stem bark of Karamunting were macerated to yield ethanolic extract. The phytochemical screening was evaluated by Harborne and Kokate methods. Antioxidant activity was evaluated by DPPH radical scavenging assay. Antibacterial activity was examined using agar well diffusion method against Propionibacterium acnes. The results showed that the ethanol extracts of twigs and stem bark samples display an ability to inhibit DPPH free radical by 82% and 88% at 50 ppm concentration. Antibacterial activity of the twigs and stem bark samples showed potent activity to inhibit the P. acnes growth with 11.3 mm and 11 mm inhibition activity. Based on the results, the twigs and stem bark of M. malabathricum plants display potential as a natural antioxidant and antibacterial agent.Downloads
References
Cappuccino, J. G., & Sherman, N. (2014). Microbiology: A Laboratory Manual, 10th Edition. New York: Rockland Community College.
Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis 3rd Edition. London, United Kingdom: Springer, 3rd Edition.
Ibrahim, N., Norha, S., Wahab, N. Z., & Ahmad, I. B. (2008). Cytotoxicity and antiviral activity of Melastoma malabathricum extracts. Malays J Appl Biol, 37(2), 53-55.
Jha, D. K., Panda, L., Ramaiah, S., & Anbarasu, A. (2014). Evaluation and comparison of radical scavenging properties of solvent extracts from Justicia adhatoda leaf using DPPH assay. Applied Biochemistry and Biotechnology, 174(7), 2413-25.
Kokate. (2000). Practical Pharmacognosy. India: Vallabh Prakashan.
Mollerup, S., Friis-Nielsen, J., Vinner, L., Hansen, T. A., Richter, S. R., Fridholm, H., . . . Hansen, A. J. (2016). Propionibacterium acnes: Disease-Causing Agent or Common Contaminant? Detection in Diverse Patient Samples by Next-Generation Sequencing. Journal of Clinical Microbiology, 54(4), 980-987.
Pokorny, J., Yanishlieva, N., & Gordon, M. (2001). Antioxidant in Food; Practical Applications. New York: Woodhead Publishing.
Roller, S. (2003). Natural Antimicrobials for the Minimal Processing of Foods. Washington DC: Elsevier Science.
Roosita, K., Kusharto, C. M., Sekiyama, M., Fachrurozi, Y., & Ohtsuka, R. (2008). Medicinal plants used by the villagers of a Sundanese community in West Java, Indonesia. Journal of Ethnopharmacology, 115(1), 72-81.
Roslen, N. A., Alewi, N. M., Ahamada, H., & Syaiful, M. (2014). Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro. Asian Pacific Journal of Tropical Biomedicine, 4(7), 545-548.
Sari, N. M., Kuspradini, H., Amirta, R., & Kusuma, I. W. (2018). Antioxidant activity of an invasive plant, Melastoma malabathricum and its potential as herbal tea product. IOP Conference Series: Earth and Environmental Science (hal. 012029). Samarinda: IOP Publishing Ltd.
Sharma, H. K., Chhangte, L., & Dolui, A. K. (2001). Traditional medicinal plants in Mizoram, India. Fitoterapia, 72(2), 146-161.
Shimizu, K., Kondo, R., Sakai, K., Takeda, N., Nagahata, T., & Oniki, T. (2001). Novel vitamin E derivative with 4-substituted resorcinol moiety has both antioxidant and tyrosinase inhibitory properties. Lipids, 36(12), 1321-1326.
Sulaiman, M. R., Somchit, M. N., Israf, D. A., Ahmad, Z., & Moin, S. (2004). Antinociceptive effect of Melastoma malabathricum ethanolic extract in mice. Fitoterapia, 75(7-8), 667-72.
Sunilson, J. A., Anandarajagopal, K., Kumari, A. V., & Mohan, S. (2009). Antidiarrhoeal Activity of Leaves of Melastoma malabathricum Linn. Indian Journal of Pharmaceutical Sciences, 71(6), 691–695.
Susanti, D., Sirat, H. M., Ahmad, F., & Ali, R. M. (2008). Bioactive constituents from the leaves of Melastoma malabathricum L. Scientific Journal of Pharmacy, 5(1), 1-8.
Susanto, D., Ruga, R., & Sudrajat. (2012). Studi Kandungan Bahan Aktif Tumbuhan Meranti Merah (Shorea leprosula Miq) Sebagai Sumber Senyawa Antibakteri. Mulawarman Scientific, 11(2), 181-190.
Taskin, T., & Taskin, D. (2017). In vitro anti-urease, antioxidant activities and phytochemical composition of Geranium purpureum. Journal of Food Measurement and Characterization, 11, 2102-2109.
Wiart, C., Rajagopal, M., Khalifah, S., Mahan, S., Ismail, S., Buckle, M., . . . Sulaiman, M. (2004). Antimicrobial screening of plants used for traditional medicine in the state of Perak, Peninsular Malaysia. Fitoterapia, 75(1), 68-73.
Zakaria, A. Z., Jaios, E. S., Omar, M. H., Rahman, S. A., Hamid, S. S., Ching, S. M., . . . Taher, M. (2016). Antinociception of petroleum ether fraction derived from crude methanol extract of Melastoma malabathricum leaves and its possible mechanisms of action in animal models. BMC Complementary and Alternative Medicine. BMC Complement Altern Med 16, 488.
Zakaria, Z. A., Mohd Nor, R. R., Kumar, G. H., Ghani, Z. A., Sulaiman, M. R., Devi, G. R., . . . Fatimah , C. A. (2007). Antinociceptive, anti-inflammatory and antipyretic properties of Melastoma malabathricum leaves aqueous extract in experimental animals. Canadian Journal of Physiology and Pharmacology, 84(12), 1291 - 1299.
