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PENGEMBANGAN FORMULA NANOSTRUCTURED LIPID CARRIER (NLC) SEBAGAI PEMBAWA MINYAK ATSIRI MELATI (Jasminum officinale L.) SERTA POTENSI AKTIVITAS ANTIOKSIDAN

DOI: https://doi.org/10.20956/mff.v27i2.27395
Article History

Submited : June 26, 2023
Published : August 31, 2023

Keywords:
Potensi penggunaan minyak atsiri sebagai bahan baku obat dan kosmetik sangat menjanjikan, salah satunya minyak atsiri melati (Jasminum officinale L.) yang telah dilaporkan memiliki berbagai aktivitas farmakologi. Namun dikarenakan karakteristiknya yang mudah menguap, rentan terdegradasi, dan kelarutannya yang rendah dalam air menyebabkan keterbatasan dalam penggunaannya. Selain itu, dalam konteks penggunaan langsung secara topikal, minyak atsiri melati (MAM) dapat menimbulkan efek iritasi pada kulit. Untuk itu, dalam penelitian ini dikembangkan suatu sistem penghantaran berbasis lipid yakni nanostructured lipid carrier (NLC) sebagai pembawa MAM. Pembuatan NLC-MAM dilakukan dengan metode emulsifikasi-sonikasi. Proses optimasi yang dilakukan meliputi optimasi konsentrasi total lipid, optimasi sistem surfaktan dan konsentrasinya, serta optimasi rasio lipid padat dan lipid cair. Selain itu, dilakukan pula optimasi proses sonikasi dengan memvariasikan tahapan sonikasi. Selanjutnya, formula optimum dikarakterisasi meliputi penentuan ukuran partikel dan distribusinya, pengukuran zeta potensial, pengamatan morfologi partikel, dan penetapan efisiensi enkapsulasi. NLC-MAM yang dihasilkan memiliki bentuk sferis dengan ukuran partikel 152,93 ± 8,18 nm, indeks polidispersitas 0,37 ± 0,05 dan zeta potensial -21,60 ± 6,75 mV. Efisiensi enkapsulasi MAM ke dalam sistem NLC yang diperoleh sebesar 97,65 ± 2,34%, dan tidak mengalami perubahan yang signifikan setelah penyimpanan selama 14 hari. Selanjutnya, potensi aktivitas antioksidan NLC-MAM dievaluasi menggunakan metode peredaman 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasil yang diperoleh menunjukan bahwa potensi aktivitas antioksidan MAM bebas sebanding dengan MAM yang dienkapsulasi ke dalam sistem NLC. Hal ini menggambarkan bahwa proses pembuatan NLC tidak mempengaruhi potensi aktivitas antioksidan MAM. Selain itu, NLC-MAM yang dihasilkan berpotensi untuk dikembangkan lebih lanjut sebagai agen terapeutik maupun kosmetik.

References

  1. Wei A, Shibamoto T. Antioxidant Activities and Volatile Constituents of Various Essential Oils. J Agric Food Chem. 2007 Mar 1;55(5):1737–42.
  2. Cimino C, Maurel OM, Musumeci T, Bonaccorso A, Drago F, Souto EMB, et al. Essential oils: Pharmaceutical applications and encapsulation strategies into lipid-based delivery systems. Pharmaceutics. 2021;13(3):1–35.
  3. Majeed H, Bian Y-Y, Ali B, Jamil A, Majeed U, Khan QF, et al. Essential oil encapsulations: uses, procedures, and trends. RSC Adv. 2015;5(72):58449–63.
  4. Ahmed N, Hanani YA, Ansari SY, Anwar S. Chapter 55 - Jasmine (Jasminum sambac L., Oleaceae) Oils. In: Preedy Flavor and Safety VRBT-EO in FP, editor. San Diego: Academic Press; 2016. p. 487–94.
  5. de Groot AC, Schmidt E. Essential Oils, Part VI: Sandalwood Oil, Ylang-Ylang Oil, and Jasmine Absolute. Dermat contact, atopic, Occup drug. 2017;28(1):14–21.
  6. Al-Snafi AS. Pharmacology and Medicinal Properties of Jasminum Officinale. Indo Am J P Sci. 2018;05(04):2191–7.
  7. Budianto BH, Sasongko ND. Composition and abundance level of pest mites in jasmine gambier (Jasminum officinale) plantation of Purbalingga, Central Java, Indonesia affected by some abiotic factors. Biodiversitas. 2022;23(10):5227–32.
  8. Hussain M, Bakhsh H, Aziz A, Majeed A, Khan IA. Comparative In vitro study of antimicrobial activities of flower and whole plant of Jasminum officinale against some human pathogenic microbes. J Pharm Altern Med. 2013;2(4).
  9. Rasheed HM, Wahid F, Qayyum R, Shah AJ, Khan T. Chemical composition and pharmacological evaluation of essential oil from jasminum officinale flowers for spasmolytic and vasodilator activities. Farmacia. 2020;68(4):722–7.
  10. Kaviani M, Maghbool S, Azima S, Tabaei MH. Comparison of the effect of aromatherapy with. 2014;19(6).
  11. Wei FH, Chen FL, Tan XM. Gas chromatographic-mass spectrometric analysis of essential oil of Jasminum officinale L var grandiflorum flower. Trop J Pharm Res. 2015;14(1):149–52.
  12. Lo CM, Han J, Wong ESW. Chemistry in Aromatherapy – Extraction and Analysis of Essential Oils from Plants of Chamomilla recutita, Cymbopogon nardus, Jasminum officinale and Pelargonium graveolens. Biomed Pharmacol J. 2020;13(3):1339–50.
  13. Tisserand R, Young R. Toxicity. Essent Oil Saf. 2014;23–38.
  14. Chauhan I, Yasir M, Verma M, Singh AP. Nanostructured lipid carriers: A groundbreaking approach for transdermal drug delivery. Advanced Pharmaceutical Bulletin. 2020.
  15. Müller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev. 2002;54(SUPPL.):131–55.
  16. Pople P V., Singh KK. Development and evaluation of colloidal modified nanolipid carrier: Application to topical delivery of tacrolimus. Eur J Pharm Biopharm. 2011;79(1):82–94.
  17. Soleimanian Y, Goli SAH, Varshosaz J, Sahafi SM. Formulation and characterization of novel nanostructured lipid carriers made from beeswax, propolis wax and pomegranate seed oil. Food Chem. 2018;244(October 2017):83–92.
  18. Loo CH, Basri M, Ismail R, Lau HLN, Tejo BA, Kanthimathi MS, et al. Effect of compositions in nanostructured lipid carriers (NLC) on skin hydration and occlusion. Int J Nanomedicine. 2013;8:13–22.
  19. Rawal SU, Patel MM. Chapter 2 - Lipid nanoparticulate systems: Modern versatile drug carriers. In: Grumezescu AMBT-LN for DT, editor. William Andrew Publishing; 2018. p. 49–138.
  20. Khalil RM, El-bary AA, Kassem MA, Ghorab MM, Ahmed MB. Solid lipid nanoparticles for topical delivery of Meloxicam: development and In Vitro characterization. Eur Sci J. 2013;4(7):24–6.
  21. Hu F-Q, Jiang S-P, Du Y-Z, Yuan H, Ye Y-Q, Zeng S. Preparation and characterization of stearic acid nanostructured lipid carriers by solvent diffusion method in an aqueous system. Colloids Surf B Biointerfaces. 2005 Nov;45(3–4):167–73.
  22. Stetefeld J, McKenna SA, Patel TR. Dynamic light scattering: a practical guide and applications in biomedical sciences. Biophys Rev. 2016;8(4):409–27.
  23. Witayaudom P, Klinkesorn U. Effect of surfactant concentration and solidification temperature on the characteristics and stability of nanostructured lipid carrier (NLC) prepared from rambutan (Nephelium lappaceum L.) kernel fat. J Colloid Interface Sci. 2017;505:1082–92.
  24. Hanieh PN, Bonaccorso A, Zingale E, Cimarelli S, Souto EB, Rinaldi F, et al. Almond oil O/W nanoemulsions: Potential application for ocular delivery. J Drug Deliv Sci Technol. 2022;72(December 2021).
  25. Wallace SJ, Li J, Nation RL, Boyd BJ. Encapsulation and Release Methodology. 2013;2(4):284–92.
  26. Marques SS, Ramos II, Fernandes SR, Barreiros L, Lima SAC, Reis S, et al. Insights on ultrafiltration-based separation for the purification and quantification of methotrexate in nanocarriers. Molecules. 2020;25(8).
  27. Tortorici S, Cimino C, Ricupero M, Musumeci T, Biondi A, Siscaro G, et al. Nanostructured lipid carriers of essential oils as potential tools for the sustainable control of insect pests. Ind Crops Prod. 2022;181(December 2021).
  28. Teeka P, Chaiyasat A, Chaiyasat P. Preparation of poly (methyl methacrylate) microcapsule with encapsulated jasmine oil. Energy Procedia. 2014;56(C):181–6.
  29. Patel DK, Kesharwani R, Kumar V. Lipid Nanoparticle Topical and Transdermal Delivery: A Review on Production, Penetration Mechanism to Skin. Int J Pharm Investig. 2019;9(4):148–53.
  30. Gunaseelan S, Balupillai A, Govindasamy K, Ramasamy K, Muthusamy G, Shanmugam M, et al. Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells. PLoS One. 2017;12(5):1–20.
-, S., Putri, P. A., & Lumintang, Y. A. (2023). PENGEMBANGAN FORMULA NANOSTRUCTURED LIPID CARRIER (NLC) SEBAGAI PEMBAWA MINYAK ATSIRI MELATI (Jasminum officinale L.) SERTA POTENSI AKTIVITAS ANTIOKSIDAN. Majalah Farmasi Dan Farmakologi, 27(2), 32-38. https://doi.org/10.20956/mff.v27i2.27395

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