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KERAGAMAN GENETIK BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA DARI Punica granatum L. DAN AKTIVITAS ANTIBAKTERINYA TERHADAP BAKTERI Staphyloccocus aureus DAN Pseudomonas aeruginosa.

DOI: https://doi.org/10.20956/mff.v26i2.19678
Article History

Submited : January 17, 2022
Published : September 2, 2022

Keywords:
Delima (Punica granatum L.) merupakan salah satu tanaman yang digunakan sebagai obat. P. granatum mempunyai berbagai khasiat, salah satunya sebagai antibakteri. Adanya efek antibakteri pada P. granatum karena mengandung senyawa antibakteri seperti, fenolik, flavonoid, dan tannin. Tujuan penelitian ini adalah mengetahui aktivitas antibakteri ekstrak daun P. granatum var Album, P. granatum var Nana dan P. granatum var Saveh Black terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeroginosa ATCC 9027 serta mengetahui tingkat kekerabatan dan keragaman geneticnya. Ekstrasi yang digunakan yaitu metode maserasi menggunakan etil asetat, etanol 96, 70, dan 30%. Hasil menunjukkan ekstrak etanol 96% P. grantum dan ekstrak etanol 70% P. granatum memiliki aktivitas penghambatan terhadap bakteri S. aureus dan bakteri P. aeruginosa, sementara ekstrak etil asetat P. granatum memiliki aktivitas penghambatan terhadap bakteri S. aureus tetapi tidak memiliki memberikan aktivitas penghambatan terhadap bakteri P. aeruginosa, sedangkan ekstrak etanol 30% P.grantum daerah Gowa dan Kediri tidak memberikan aktivitas penghambatan terhadap S. aureus dan P. aeruginosa. Analisis RAPD dan dendogram menunjukkan bahwa hubungan kekerabatan antara dua kelompok P. granatum berkisar 0,65-0,72. Hal ini menunjukkan bahwa keragaman genetik antara ketiga varietas P. granatum dengan empat daerah yang berbeda cukup rendah meskipun memiliki fenotip yang berbeda.

References

  1. Jurenka, Julie. 2008. Therapeutic Application of Pomegranate (Punica granatum L.): A Review. Alternative Medicine Review, Vol. 13 (2), pp. 128-144.
  2. Hardana H. Efrida W. 2015. Ekstrak Buah Delima Sebagai Antibiotik Pengobatan Infeksi MRSA. Majority. Vol 4 (9), pp. 83-87.
  3. Ravinder K., Sonia K., Poonam S. 2018. Antimicrobial and antioxidant potential of pomegranate (Punica granatm L.) Peel. International Journal of Chemical Studies. Vol 6 (5), pp. 3441-3449.
  4. Yaser N., Reza G., Reza M., Majid F. 2018. Antibacterial Activity of Pomegranate (Punica granatum L.) Seed and Peel Alcoholic Extracts on Staphylococcus aureus and Pseudomonas aeruginosa Isolated From Health Centers. J Appl Biotechnol Rep. Vol 5 (1), pp. 32-36.
  5. Al-Zoreky NS. 2009. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol. Vol 134 (3), pp. 244-248.
  6. Dahham SS, Ali MN, Tabassum H, Khan M. 2010. Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). Am Eurasian J Agric Environ Sci. Vol. 9 (3), pp. 273-281.
  7. Dahanukar S A., Kulkarni R A., Rege N N. 2000. Pharmacology of medicinal plants and natural products. Indian journal of pharmacology. 32(4), pp. 81-118.
  8. Amine A., Mohamed A., Aimen A. 2020. Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves. Scientifica. DOI: 10.1155/2020/8271203.
  9. Ercisli S, Gadze J, Agar G, Yildirim N, Hizarci Y .2011. Genetic relationships among wild pomegranate (Punica granatum) genotypes from Coruh Valley in Turkey. Genet Mol Res. Vol 10 (1), pp. 459-464.
  10. Weising K., Nybom H., K Wolff., and W Meyer. 1995. DNA Fingerprinting in Plants and Fungi. Boca Rato: CRC Press.
  11. Anggereini E, 2008. Random Amplified Polymorphic DNA (RAPD), Suatu Metode Analisis DNA dalam Menjelaskan Berbagai Fenomena Biologi. Biospecies. Vol. 1 (2), pp. 73-76.
  12. Widyatmoko, A.Y.P.B.C., Lejo, E.S., Prasetyaningsih, A & Rimbawanto, A. 2010. Keragaman Genetik Populasi Araucaria cunninghamii Menggunakan Penanda RAPD (Random Ampified Polymorphic DNA). Jurnal Pemuliaan Tanaman. Vol 4 (2), pp. 63–77.
  13. Jahir A and Sonali H. 2011. Antibacterial Properties of Punica granatum Peels. International Journal of Applied Biology and Pharmaceutical Technology. Vol. 2 (3), pp. 23-27.
  14. Braga LC, Shupp JW, Cummings C. 2005. Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol. Vol. 96 (1-2), pp. 335-339.
  15. Falah S., Hasim., Ayunda R. D., Faridah D. N. 2015. Potential of Lemongrass extract (Cymbopogon citratus) as prevention for oil oxidation. Journal of Chemical and Pharmaceutical Research. Vol. 7 (10), pp. 55-60.
  16. Priya V, Mallika J, Surapaneni KM, Saraswathi P, Chandra SG. 2010. Antimicrobial activity of pericarp extract of Garcinia mangostana Linn. International Journal of Pharma Sciences and Research. Vol. 1 (8), pp. 278-281.
  17. Pelczar, Michael J., dan Chan, E. C. S., 1986, Dasar-Dasar Mikrobiologi, Universitas Indonesia, UI-Press, Jakarta. 190-191.
  18. Jawetz, E., Melnick, J. L., Adelberg, E. A., 2001, Mikrobiologi Kedokteran, Edisi XXII, diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga, 205-209, Penerbit Salemba Medika, Jakarta.
  19. Salni. Hanifa., W e d y a , R., 2011, Isolasi Senyawa Antibakteri Dari Daun Jengkol (Pithecolobium lobatum Benth) dan Penentuan Nilai KHM-nya. Jurnal Penelitian Sains. Vol. 14 (1), pp. 14109.
  20. Fateh MV, Ahmed S, Ali M, Bandyopadhyay S. 2013. A review on the medicinal importance of pomegranate. J Pharm sci. Vol. 3 (4), pp. 23-25.
  21. Heber D, Schulman RN, Seeram NP. Pomegranates: ancient roots to modern medicine. CRC Press; 2006.
  22. Satomi H., umemura K., Ueno A. 1993. Carbonic Anhydrase Inhibitors from The Pericarps of Punica granatum L. Biol Pharm Bull. Vol 16, pp. 787-790.
  23. Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D. 2007. Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. Planta Med. Vol. 73 (5), pp. 461 467. doi:10.1055/s-2007-967167.
  24. Voravuthikunchai SP, Sririrak T, Limsuwan S, Supawita T, Iida T, Honda T. 2005. Inhibitory effects of active compounds from Punica granatum pericarp on verocytotoxin production by enterohemorrhagic Escherichia coli O157: H7. J Health Sci. Vol. 51 (5), pp. 590-596. doi:10.1248/jhs.51.590
  25. Prashanth D, Asha MK, Amit A. 2001. Antibacterial activity of Punica granatum. Fitoterapia. Vol. 72 (2), pp. 171-173. doi:10.1016/S0367326X(00)00270-7.
  26. Rachida B., Imene Y., sarah B. 2020. Phytochemical Analysis, Antibacterial and Antioxidant Activities of Pomegranate (Punica granatum L) Peel extracts. Int J Biosci. Vol 16 (6), pp. 35-44.
  27. Hossain T., Furhatun N., Assadujaman. 2021. A Review Study on The Pharmacological Effect And Mechanism O Action Of Tannins. European Journal of Pharmaceutical and Medical Reseacrh. Vol 8 (8), pp. 5-10.
  28. Noventi, W and N. Carolia. 2016. Potensi Ekstrak Daun Sirih (Piper betle L.) sebagai alternatif Acne vulgaris. Majority. Vol 5 (1), pp. 140-145.
  29. Volk WA. 1992. Basic Microbiology (7 ed). New York: Harper Collins Publisher.
  30. Jawetz E, JJ Melnick & EA Adelberg. 2001. Mikrobiologi Kedokteran Buku 1. Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga (Penterjemah). 2005. Jakarta: Salemba Medika.
  31. Larekeng S. Selection of Dominant and Co-dominant Markers for Red Wood (Pterocarpus indicus Willd) Polymorphism from Five Provenances in East Nusa Tenggara. In: 1st International Conference on Science and Technology, ICOST. 2019.
  32. Larekeng SH, Dermawan R, Iswoyo H, Mustari K. 2019. RAPD primer screening for amplification on Katokkon pepper from Toraja, South Sulawesi, Indonesia. IOP Conf Ser Earth Environ Sci ;270. 012-023.
  33. Gunawan. Red Wood DNA Amplification (Pterocarpus indicus Willd) on some Provenances in East Nusa Tenggara. Hasanuddin University; 2019.
  34. Olivier M., Meehl MA, Lust G. 1999. Random Amplified Polymorphic DNA Sequences as Markers for Canine Genetic Studies. The journal of heredity. Vol 90 (1).
  35. Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy od Sciences 76:5269–5273. https://doi.org/10.1073/pnas.76.10.5269
  36. Campbell, Reece & Mitchell L. 2008. Biologi. Edisi Kedelapan Jilid 1. Jakarta : Erlangga.
Rifdah Anggrini Zabir, Dr. Herlina Rante, M.Si., Apt, Prof. Dr. Gemini Alam, M.Si., Apt, & Dr. Siti Halimah Larekeng, SP., MP. (2022). KERAGAMAN GENETIK BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA DARI Punica granatum L. DAN AKTIVITAS ANTIBAKTERINYA TERHADAP BAKTERI Staphyloccocus aureus DAN Pseudomonas aeruginosa. Majalah Farmasi Dan Farmakologi, 26(2), 63-68. https://doi.org/10.20956/mff.v26i2.19678

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