Ablasi Tangkai Mata Mempercepat Pematangan Ovari Lobster Pasir (Panulirus homarus) pada Musim Pemijahan

Authors

  • Yudha Trinoegraha Adiputra
  • Muhammd Zairin Junior
  • Muhammad Agus Suprayudi
  • Wasmen Manalu
  • Widanarni Widanarni

Abstract

Musim pemijahan alami lobster pasir di perairan Pesisir Barat Lampung terjadi pada Juli-September yang ditandai dengan hasil tangkapan induk betina yang membawa telur mendominasi saat ditangkap. Pelarangan penangkapan lobster pasir ukuran kecil diduga akan menganggu produksi penangkapan sehingga perlu dimulai pembenihan yang memperlukan teknik pemeliharaan dan maturasi ovari induk betina lobster pasir hasil tangkapan alam. Tujuan penelitian ini mempelajari cara pemeliharaan induk dan pengaruh ablasi satu tangkai mata pada maturasi ovari lobster pasir (Panulirus homarus). Empatpuluh delapan ekor lobster pasir digunakan dan dipisahkan menjadi 2 kelompok yaitu ablasi satu tangkai mata dan tanpa ablasi tangkai mata. Tiga ekor diambil dari setiap kelompok pada hari ke-1:3:7:10:14:21 dan 27 untuk evaluasi perubahan anatomi ovari dan gonadosomatik indeks (GSI). Hasil penelitian menunjukkan seleksi selama masa adaptasi induk dapat meniadakan kematian induk karena ablasi mata. Anatomi ovari menunjukkan terjadinya maturasi ovari yang meningkat dari matang awal menjadi matang berlebih dengan ablasi mata pada hari ke-3 setelah ablasi sampai hari ke-27. Berbeda dengan perlakuan diatas, induk betina tanpa ablasi mata tidak dapat mematangkan ovari secara spontan. Setelah 27 hari, ovari secara serentak mencapai matang berlebih yang diasumsikan karena nutrien dari pakan yang diberikan dapat mendukung maturasi ovari. Perbedaan GSI antara perlakuan ablasi satu tangkai mata berbeda nyata (P<0,05) dengan tanpa ablasi tangkai mata. Hal ini menunjukkan hilangnya hormon penghambatan vitelogenesis (VIH) dan terpacunya hormon stimulasi vitelogenesis (VSH) karena ablasi tangkai mata. Studi merekomendasikan adaptasi untuk seleksi induk, ablasi untuk memperoleh maturasi ovari yang singkat dibandingkan dengan tanpa ablasi mata meskipun dengan nutrien pakan yang berkualitas. Kata Kunci: ablasi mata, GSI, lobster pasir, maturasi ovari, musim pemijahan 

Downloads

Download data is not yet available.

References

Adiputra, Y.T., Hudaidah, S., Saputra, A.B., Halim, D.N.A., dan Firmansyah. 2018. Pengembangan perikanan lobster di Provinsi Lampung. Jurnal Kelitbangan Inovasi Pembangunan 6:49-59.

Asusena, A.-C. J., Carlos, S.-H. J., Arturo, F.-C. J., and Genaro, D.-P. 2012. The effects of eyestalk ablation on the reproductive and immune function of female Macrobrachium americanum. J. Aquac. Res. Development 3:156. doi:10.4172/2155-9546.1000156.

Bhujel, R.C. 2008. Statistics for Aquaculture. Wiley-Blackwell, Ames. Iowa. 222 pp.

Bray, W.A., and Lawrence, A.D.1992.Reproduction of Penaeus species in captivity, page.93-150. In A.W.Fast and L.J.Lester (eds) Marine Shrimp Culture: Principles and Practices. Elsevier Science B.V. Netherlands.

Chang, Y.-J., Sun,C.-L., Yong, C., Yeh, S.-Z., and Chiang, W.-C. 2007. Reproductive biology of the spiny lobster, Panulirus penicillatus, in the southeastern coastal waters off Taiwan. Mar.Biol. 151:553-564.

Chimsung N.2014.Maturation diets for black tiger shrimp (Penaeus monodon) broodstock:a review.Songklanakarin J. Sci.Technol.36:265-273.

Fernandez, R., and Radhakhrishnan.2016. Effect of bilateral eyestalk ablation on ovarian development and moulting in early and late intermoult stages of female spiny lobster Panulirus homarus (Linnaeus, 1758). Invertebrate Reproduction & Development 60:238-242.

Girsang, E., Kristanto, A.H., Hadi, W., dan Mardlijah, S. 2014. Karakterisasi biometrik lobster (Panuliru homarus) dari beberapa lokasi. Dalam Lokakarya Nasional Pengelolaan dan Perlindungan Sumber Daya Genetik di Indonesia: Manfaat Ekonomi untuk Mewujudkan Ketahanan Nasional. Halaman 298-306.

Harrison, K.E. 1990. The role of nutriton in maturation, reproduction and embryonic development of decapod crustaceans: a review. Journal of Shellfish Research 9(1):1-28.

Hesni, M.A., Shabanipour, N., Atabati., and Bitarat, A. 2008. Influence fo eyestalk ablation and temperature on molting and mortality of narrow-clawed crayfish (Astacus leptodactylus). Turkish Journal of Fisheries and Aquatic Sciences 8:219-223.

Hosamani, N., Pamuru, R.R., and Pamanji, S.R.2016. Natural and induced (eyestalk ablation) molt cycle in freshwater rice field crab Oziothelphusa senex senex. J. Aquac. Res. Development 7:424. doi:10.4172/2155-9546.1000424.

Huberman, A.2000.Shrimp endocrinology.Aquaculture 191:191-208.

Juinio-Meñez, M.A., and Ruinata, J.1996.Survival, growth and food conversion efficiency of Panulirus ornatus following eyestalk ablation. Aquaculture 146:225-235.

Kizhakudan, J.K. 2013. Effect of eyestalk ablation on moulting and growth in the mudspiny lobster Panulirus polyphagus (Herbst, 1793) held in captivity. Indian J. Fish. 60(1):77-81.

Kizhakudan, J.K. 2014. Reproductive biology of the female shoved-nosed lobster Thenus unimaculatus (Burton and Davie, 2007) from north-west coast of India. Indian Journal of Marine Science 43(6):927-935.

Makinouchi S., and Honculada-Primavera J.1987.Maturation and spawning of Penaeus indicus using different ablation methods.Aquaculture 62:73-81.

Marsden, G., Mather, P., and Richardson, N. 2007. Captivity, ablation and starvation of the prawn Penaeus monodon affects protein and lipid content in ovary and hepatopancreas tissues. Aquaculture 271:507-515.

Maynard D.M., and Dingle H.1963.An effcet of eyestalk ablation on anttenular function in the spiny lobster, Panulirus argus. Zeitschrift fur vergleichende Physiologie 46:515-540.

Melville-Smith R., Goosen P.C., and Stewart T.J. 1995.The spiny lobster Jasus lalandii (H.Milne Edwards, 1873) off the South Africa Coast:inter-annual variations in male growth and female fecundity.Crustaceana 68:174-183.

Minagawa M., and Higuchi S.1997.Analysis of size gonadal maturation, and functional maturity in the spiny lobster Panulirus japonicus (Decapoda:Palinuridae).Journal of Crustacean Biology 17:70-80.

Murugesan, R., Soundarapandian, P., and Manivannan, K. 2008. Effect of unilateral eyestalk ablation on the biochemical change of edible portunid crab Charybdis lucifera (Fabricius). Journal of Fisheries and Aquatic Science 3(1):82-86.

Nagaraju, G.P.C.2011.Reproductive regulators in decapod crustaceans:an overview. The Journal of Experimental Biology 214:3-16.

Nakamura K.1990.Maturation of the spiny lobster Panulirus japonicus.Mem. Fac. Fish. Kagishima Univ. 39:129-135.

Nan, F.-H., Sheen, S.-S., Cheng, Y.-S., and Chen, S.-N.1995. The effects of eyetalk ablation on oxygen consumption and ammonia-N excretion of juvenile shrimp Penaeus monodon. Zoological Studies 34(4):265-269.

Paran, B.C., Fierro, I.J., and Tsukimura, B. 2010. Stimulation of ovarian growth by methyl farnasoate and eyestalk ablation in penaeoidean model shrimp, Sicyonia ingentis Burkenroad, 1938. Aquaculture Research 41:1887-1897.

Pervaiz, P.A., and Sikdar, M. 2014. Influence of bilateral eyestalk ablation on gonads of freshwater prawn, Macrobrachium dayanum. Journal of Environmental Biology 35:821-825.

Pillai, B.R., Sahoo, L., Sahu, S., Vijaykumar, S.M., and Sahu, S. 2010. Effect of unilateral eyestalk ablation on ovarian maturation and occurance of berried females in Macrobrachium rosenbergii (de Man). Indian J. Fish. 57(4):77-80.

Prasertlux, S., Sittikankaew, K., Chumtong, P., Khamnamtong, B., and Klinbunga, S. 2011. Molecular characterization and expression of Prostaglandin reductase 1 gene and protein duirng ovarian development of the giant tiger shrimp Penaeus monodon. Aquaculture 322-323:134-141.

Quackenbush, L.C.1993. Lobster reproduction: a review. Crustaceana 67(1):82-94.

Quackenbush, L.S., and Herrnkind, W.F. 1981. Regulation of molt and gonadal evelopment in the spiny lobster Panulirus argus (Crustacea: Palinuridae):effect of eyestalk ablation. Comp. Biochem.Physiol 69A:523-527.

Quackenbush, L.S., and Herrnkind, W.F. 1983. Partial characterization of eyestalk hormones controlling molt and gonadal development in the spiny lobster Panulirus argus. Journal of Crustacean Biologi 3(1):34-44.

Radhakrishnan, E.V., and Vijayakumaran, M.1984. Effect of eyestalk ablation in the spiny lobster Panulirus homarus (Linnaeus):3.On gonadal maturity. Indian Journal of Fisheries 31:209-216.

Radhakrishnan E.V.2015.Review of Prospects for Lobster Farming. In Advances in Marine and Brackishwater Aquaculture. Perumal S, Thirunavukkarasu A.R & Pachiappan P.(Eds).Springer.India. p.173-185

Remany, M.C., Kirubagaran, R., Dharani, G., Vinithkumar, N.V., Inbakandan, D., Magesh, P. D., Senthil, M.T., Dalmin G., Mary, L.T., Babu, T.D., Nair, K.V.K., Sampath, V., Subramoniam, T., and Ravindran, M. 2002. Effect of eyestalk ablation on growth and gonadal maturation in the Indian spiny lobster Panulirus homarus (Linnaeus, 1758) under captivity. Proc.XX.Symp. Reprod.Biol.Comp. Endocrinol.Held in Jan 7-9, 2002 at Bharathidasan University, Tiruchirappalli. 155-156 pp.

Sainz-Hernández, J.C., Racotta, I.S., Dumas, S., and Hernández-López, J.2008. Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables. Aquaculture 283:188-193.

Santiago Jr, A.C.1977. Successful spawning of cultured Penaeus monodon Fabricius after eyestalk ablation.Aquaculture 11:185-196.

Silva, J.R.F., and da Cruz-Landim, C.2006. Macroscopic aspects and scanning electron microscopy of the ovaries of the spiny lobster Panulirus (Crustacea:Decapoda). Braz. J. Morphol. Sci.23 (3-4): 479-486.

Stella, V.S., Greco, L.S.L., and Rodríguez, E.M. 2000. Effects of eyestalk ablation at different times of the year on molting and reproduction of the estaurine grapsid crab Chasmagnathus granulata (Decapoda, Brachyura). Journal of Crustacean Biology 20(2):239-244.

Subramoniam T., Kirubagaran R.2010.Endocrine regulation of vitellogenesis in lobsters. J. Mar. Biol. Ass. India 52:229-236.

Subramoniam T.2011. Mechanism and control of vitellogenesis in crustaceans.Fish. Sci. 77:1-21.

Sudhakar, M., Manivannan, K., Soundarapandian, P., and Ananthan, G.2009. Effect of unilateral ablation on the biochemical changes of edible portunidae crab Portunus sanguinolentus (Herbst). Middle-East Journal of Scientific Research 4(3):153-157.

Swetha, C.H., Sainath, S.B., Reddy, P.R., and Reddy, P.S. 2011. Reproductive endocrinology of female crustaceans:perspective and prospective. J. Aquac. Res. Development S3:001. doi:10.4172/2155-9910.S3-001.

Shyamal S., Sudha K., Gayathri N., & Anilkumar G.2014.The Y-organ secretory activity fluctuates in relation to season of molt and reproduction in the brachyuran crab, Metopograpsus messor (Grapsidae):ultrastructural and immunohistological study. General and Comparative Endocrinology 196:81-90.

Tamone, S.L., Adams, M.M., and Dutton, J.M. 2005. Effect of eyestalk-ablation on circulating ecdysteroids in hemolymph of snow crabs, Chionoecetes opilio:Physiological evidence for a terminal molt. Integr.Comp.Biol. 45:166-171.

Thongda W., Chung J.S., Tsutsui N., Zmora., and Katenta A.2015.Seasonal variations in reproductive activity of the blue crab, Callinectes sapidus:vitellogenin expression and levels of vitellogenin in the hemolymph during ovarian development.Comparative Biochemistry and Physiology, Part A 179:35-43.

Varalakshmi, K.N., and Reddy, R. 2010. Effects of eyestalk ablations on growth and ovarian maturation of the freshwater prawn Macrobrachium lanchesteri (de Man). Turkish Journal of Fisheries and Aquatic Science 10:403-410.

Venkitaraman, P.R., Jayalakshmy, K.V., and Balasubramanian, T. 2010. Effect of eyestalk ablation on moulting and growth in the penaeid shrimp Metapenaeus monoceros (Fabricius, 1798). Indian J. Fish 57(2):25-32.

Verghese, B., Radhakrishnan, E.V., and Padhi, A. 2008. Effect of moulting, eyestalk ablation, starvation and transportation on the immune response of the Indian spiny lobster, Panulirus homarus. Aquaculture Research 39:1009-1013.

Vijayakumaran, M., and Radhakrishnan, E.V.1984. Effect of eyestalk ablation in the spiny lobster Panulirus homarus (Linnaeus):2.On food intake and conversion. Indian Journal of Fisheries 31:148-155.

Vijayakumaran M., Maharajan A., Rajalakshmi S., Jayagopal P., Subramanian M.S., and Remani C.2012.Fecundity and viability of eggs in wild breeders of spiny lobster, Panulirus homarus (Linnaeus, 1758), Panulirus versicolor (Latrielle, 1804) and Panulirus ornatus (Fabricius, 1798). J. Mar.Biol.Ass.India 54(2):18-22.

Wu, J., Kang, X., Mu, S., and Tian, Z.2013. Effect of eyestalk ablation in Eriocheir sinensis on physiological and biochemical metabolism. Agricultural Sciences 4:25-29.

Downloads

Published

2018-07-22

How to Cite

Adiputra, Y. T., Junior, M. Z., Suprayudi, M. A., Manalu, W., & Widanarni, W. (2018). Ablasi Tangkai Mata Mempercepat Pematangan Ovari Lobster Pasir (Panulirus homarus) pada Musim Pemijahan. Prosiding Simposium Nasional Kelautan Dan Perikanan, 5. Retrieved from https://journal-old.unhas.ac.id/index.php/proceedingsimnaskp/article/view/4662