Advance Search

AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

DOI: https://doi.org/10.20956/mff.v24i2.10074
Article History

Submited : May 21, 2020
Published : August 31, 2020

Garcinia kydia Roxburgh merupakan salah satu spesies dari genus garcinia, yang secara khusus belum banyak diketahui aktivitas senyawa bioaktif, namun pada penelitian sebelumnya terbukti berpotensi sebagai agen penghambat α-glukosidase. Pada penelitian ini, akan dilakukan investigasi aktivitas antioksidan terhadap berbagai fraksi yang diperoleh dari daun G. kydia Roxb. Metode penelitian dilakukan dengan menentukan aktivitas antioksidan melalui analisis in-vitro menggunakan microplete reader dengan metode radical scavenging (Metode DPPH) dan ferric reducing antioxidant power (Metode FRAP). Hasil menunjukkan bahwa fraksi yang paling aktif dari ekstrak etil asetat adalah FEA8 (DPPH) dan FEA7 (FRAP), sedangkan fraksi paling aktif dari ekstrak metanol adalah FMT4 (DPPH) dan FMT6 (FRAP). Aktivitas antioksidan tertinggi di antara empat fraksi adalah FEA7 dan FEA8. Aktivitas fraksi FEA7 dan FEA8 ditentukan oleh nilai EC50, sehingga diperoleh masing-masing 9,73 dan 9,26 μg/mL (DPPH); 10,28 dan 13,94 μg/mL (FRAP). Garcinia kydia Roxb. berpotensi sebagai agen antioksidan dengan mekanisme yang berbeda. Sehingga hasil penelitian terkait terdapatnya potensi antioksidan tersebut dapat memberikan alternatif terapi diabetes yang disertai komplikasi stres oksidatif. 

References

  1. Gontijo VS, De Souza TC, Rosa IA, Soares MG, Da Silva MA, Vilegas W, et al. Isolation and evaluation of the antioxidant activity of phenolic constituents of the Garcinia brasiliensis epicarp. Food Chem [Internet]. 2012;132(3):1230–1235. Available from: http://dx.doi.org/10.1016/j.foodchem.2011.10.110
  2. Hemshekhar M, Sunitha K, Santhosh MS, Devaraja S, Kemparaju K, Vishwanath BS, et al. An overview on genus garcinia: Phytochemical and therapeutical aspects. Phytochem Rev. 2011;10(3):325–351.
  3. Fouotsa H, Lannang AM, Mbazoa CD, Rasheed S, Marasini BP, Ali Z, et al. Xanthones inhibitors of α-glucosidase and glycation from Garcinia nobilis. Phytochem Lett. 2012;5(2):236–239.
  4. Mahamodo S, Rivière C, Neut C, Abedini A, Ranarivelo H, Duhal N, et al. Antimicrobial prenylated benzoylphloroglucinol derivatives and xanthones from the leaves of Garcinia goudotiana. Phytochemistry [Internet]. 2014;102:162–168. Available from: http://dx.doi.org/10.1016/j.phytochem.2014.03.006
  5. Wu YP, Zhao W, Xia ZY, Kong GH, Lu XP, Hu QF, et al. Three new xanthones from the stems of Garcinia oligantha and their anti-TMV activity. Phytochem Lett [Internet]. 2013;6(4):629–632. Available from: http://dx.doi.org/10.1016/j.phytol.2013.08.006
  6. Supasuteekul C, Nonthitipong W, Tadtong S, Likhitwitayawuid K, Tengamnuay P, Sritularak B. Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis. Brazilian J Pharmacogn [Internet]. 2016;26(3):312–320. Available from: http://dx.doi.org/10.1016/j.bjp.2016.01.007
  7. Mohamed GA, Al-Abd AM, El-halawany AM, Abdallah HM, Ibrahim SRM. New xanthones and cytotoxic constituents from Garcinia mangostana fruit hulls against human hepatocellular, breast, and colorectal cancer cell lines. J Ethnopharmacol [Internet]. 2017;198:302–312. Available from: http://dx.doi.org/10.1016/j.jep.2017.01.030
  8. Parveen M, Azaz S, Zafar A, Ahmad F, Silva MR, Silva PSP. Structure elucidation, DNA binding specificity and antiproliferative proficiency of isolated compounds from Garcinia nervosa. J Photochem Photobiol B Biol [Internet]. 2017;167:176–188. Available from: http://dx.doi.org/10.1016/j.jphotobiol.2016.12.035
  9. Wahyuni FS, Ali DAI, Lajis NH, Dachriyanus. Anti-inflammatory activity of isolated compounds from the Stem Bark of Garcinia cowa Roxb. Pharmacogn J. 2017;9(1):55–57.
  10. Dontha S. A review on antioxidant methods. Asian J Pharm Clin Res. 2016;9(2):14–32.
  11. Muriithi E, Bojase-Moleta G, Majinda RRT. Benzophenone derivatives from Garcinia livingstonei and their antioxidant activities. Phytochem Lett [Internet]. 2016;18:29–34. Available from: http://dx.doi.org/10.1016/j.phytol.2016.08.019
  12. Tian DS, Gu W, Wang LP, Huang LJ, Rao Q, Huang T, et al. Cytotoxic and anti-oxidant biphenyl derivatives from the leaves and twigs of Garcinia multiflora. Phytochem Lett [Internet]. 2017;19:132–135. Available from: http://dx.doi.org/10.1016/j.phytol.2016.12.021
  13. Carrillo-Hormaza L, Ramírez AM, Quintero-Ortiz C, Cossio M, Medina S, Ferreres F, et al. Comprehensive characterization and antioxidant activities of the main biflavonoids of Garcinia madruno: A novel tropical species for developing functional products. J Funct Foods [Internet]. 2016;27:503–516. Available from: http://dx.doi.org/10.1016/j.jff.2016.10.001
  14. Taher M, Susanti D, Rezali MF, Zohri FSA, Ichwan SJA, Alkhamaiseh SI, et al. Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f. Asian Pac J Trop Med [Internet]. 2012;5(2):136–141. Available from: http://dx.doi.org/10.1016/S1995-7645(12)60012-1
  15. Biloa Messi B, Ho R, Meli Lannang A, Cressend D, Perron K, Nkengfack AE, et al. Isolation and biological activity of compounds from Garcinia preussii. Pharm Biol. 2014;52(6):706–711.
  16. Putri NL, Elya B, Puspitasari N. Antioxidant activity and lipoxygenase inhibition test with total flavonoid content from Garcinia kydia roxburgh leaves extract. Pharmacogn J. 2017;9(2):280–284.
  17. Elya B, Katrin, Mun’im A, Hasiholan A, Marlin I, Mailandari M. Antioxidant activities of leaves extracts of three species of Garcinia. Int J Med Arom Plants. 2012;2(4):691–693.
  18. Martha S, Elya B, Hanafi M. Comparison of inhibitory activity against the α-glucosidase enzymes in the extracts and fractions from leaves of the Garcinia kydia Roxburgh. Asian J Pharm Clin Res. 2017;10(7):401–404.
  19. Sanseera D, Liawruangrath B, Pyne SG, Liawruangrath S. Determination of antioxidant and anticancer activities together with total phenol and flavonoid contents of Cleidion javanicum Bl. And Bridelia retusa (L.) A. Juss. Chiang Mai J Sci. 2016;43(3):534–545.
  20. Jiangseubchatveera N, Liawruangrath S, Teerawutgulrag A, Santiarworn D, Pyne SG, Liawruangrath B. Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff. Chiang Mai J Sci. 2017;44(1):193–202.
  21. Juma I. Assessment of antioxidant potentials of the wild and domesticated saprophytic edible mushrooms from Tanzania. Curr Res Environ Appl Mycol. 2016;6(1):1–10.
  23. Nyau V, Prakash S, Rodrigues J, Farrant J. Antioxidant activities of Bambara groundnuts as assessed by FRAP and DPPH assays. Am J Food Nutr [Internet]. 2015;3(1):7–11. Available from: http://pubs.sciepub.com/ajfn/3/1/2/index.html
  24. Maizura M, Aminah A, Aida WMW. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. Int Food Res J. 2011;18(2).
  25. Sousa A, Araújo P, Azevedo J, Cruz L, Fernandes I, Mateus N, et al. Antioxidant and antiproliferative properties of 3-deoxyanthocyanidins. Food Chem. 2016;192:142–148.
  27. Pereira ACH, Lenz D, Nogueira BV, Scherer R, Andrade TU, Da Costa HB, et al. Gastroprotective activity of the resin from Virola oleifera. Pharm Biol [Internet]. 2017;55(1):472–480. Available from: http://dx.doi.org/10.1080/13880209.2016.1251467
  28. Chen Z, Bertin R, Froldi G. EC50 estimation of antioxidant activity in DPPH* assay using several statistical programs. Food Chem [Internet]. 2013;138(1):414–420. Available from: http://dx.doi.org/10.1016/j.foodchem.2012.11.001
  29. Maesaroh K, Kurnia D, Al Anshori J. Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. Chim Nat Acta. 2018;6(2):93.
  30. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J [Internet]. 2013;21(2):143–152. Available from: http://dx.doi.org/10.1016/j.jsps.2012.05.002
  31. Molyneux P. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating anti-oxidant activity. Songklanakarin J Sci Technol. 2004;26(2):211–219.
  32. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199–1200.
  33. Kumar S, Pandey AK. Review Article Chemistry and Biological Activities of Flavonoids: An Overview. Sci World J. 2013;
  34. Ozgen S, Kilinc OK, Selamoglu Z. Antioxidant Activity of Quercetin: A Mechanistic Review. Turkish J Agric -Food Sci Technol [Internet]. 2016;4(412):1134–1138. Available from: www.agrifoodscience.com,
  35. Csepregi K, Neugart S, Schreiner M, Hideg É. Comparative evaluation of total antioxidant capacities of plant polyphenols. Molecules. 2016;21(2):1–17.
Martha, S., Elya, B., & Hanafi, M. (2020). AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh. Majalah Farmasi Dan Farmakologi, 24(2), 37-41. https://doi.org/10.20956/mff.v24i2.10074

Downloads

Download data is not yet available.
Fulltext
PDF