ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE Eucheuma cottonii AND Eucheuma spinosum
DOI:
https://doi.org/10.20956/mca.v17i2.1123Abstract
Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent.Downloads
References
Angka S.L. &Suhartono M.T. (2000). Marine Biotechnology results. Center for Coastal and Marine Resource. Bogor: IPB. Edition I. 149p.
Atmadja, W.S., Sulistijo., Kadi, A., Sahari., 1996. Intro Type Seaweed in Indonesia. 30p. LIPI, Jakarta.
Chow S.T., Chao W.W. & Chung Y.C. (2003). Antioxidative Activity and Safety of 50% Ethanolic Red Bean Extract (Phaseolusraditus L. VarAurea). J. Food Science, 68(1):21-25.
Cristiane M.R., De Souza., C.T. Marques., D.C.M. Guerra., F.R.F. Da Silva., Rocha., dkk. (2006). Antioxidant Activities OfSulphated Polysaccharides From Brown And Red Seaweed. Springer Science and Business Media B.
Hambali E., Ani, S., Wadli (2004). Make a variety of processed seaweed. Publisher Swadaya. Jakarta.
Hidayat A. 2001. Seaweed Cultivation. Publisher Usaha Nasimal. Surabaya.
Hijaz M.N. (2009). Antioxidant Activity Test Red Algae type of carrageenan in Eucheuma spinosum and Gracilaria verrucosa (Tesis). Universitas Islam Negeri Malang. Malang
Kong, F., Zhang, M., Liao, S., Yu, S., Chi, J., Wei, Z., 2010. Antioxidant Activity of Polysaccharide-enriched Fractions Extracted from Pulp Tissue of Litchi Chinensis Sonn, Molecules, 15: 2152-2165.
Mahendran S. &Saravanan S. (2013).Purification And In Vitro antioxidant Activity Of Polysaccharide Isolated From Green Seaweed Caulerpa racemosa. International Journal of Pharma and Bio Sciences, 4(4):(B) 1214-1227.
Meyer B. N., Ferrigni N. R., Putman J. E., Jacbsen L.B., Nicols D. E., &Mc Laughlin J. L. (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Plant Medica.
Molyneux P. (2004). The Use of The Stable Free Radical Diphenyl picyrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin. J. Sci. Technol, 26(2):211-219.
Murdinah., Nurbaity., Apriani., Nurhayati., dan Subaryono SP. 2012 To Make of jelly from red algae Gracilaria sp. Publisher Swadaya. Jakarta.
Nihiati I. A., Hertiani T. &Rohman T.(2008). Rhizome Extract Antioxidant Power Extract Ethanol From Rhizoma (Boesenbergia Pandurata) with Free Radical arrest Method of DPPH (1,1-difenil-2-pikrilhidrazil) Method. Publisher Majalah Obat Tradisional,13 (45): 101– 108.
Oke J.M. & Hamburger M.O. (2002). Screening of Some Nigerian Medicinal Plants for Antioxidant Activity Using DPPH Radical. Afr. J. Biomed Res, 5: 77-79.
Solomon RDJ., Santhi VS. (2008). Purification of bioactive natural product against human microbial pathogens from marine seaweed Dictyota acutiloba. J. Ag. World. J Microbiol. Biotechnol. 24:1747-1752.
Souza B.W.S., Cerqueira M.A., Bourbon A.I., Pinheiro A.C., Martins J.T., Teixeira J.A., dkk. (2012). Chemical Characterization, Antioxidant Activity of Sulfated Polysaccharide from The Red Seaweed Gracilariabirdiae. Food Hydrocolloids,27: 287-292.
Wikanta T., Januar H.I. &Nursid M. (2005). Uji Aktivitas Antioksidan, Toksisitas dan Sitotoksisitas Ekstrak Alga Merah Rhodymenia palmata. Jurnal Penelitian Perikanan Indonesia, 11 (4):41-49.
Ye, H., Wang, K., Zhou, C., Liu, J., Zeng, X., 2008. Purification, Antitumor and Antioxidant activities in Vitro of Polysaccharides from The Brown Seaweed Sargassum pallidum. Food Chemistry. 111: 428-432.
