Penggunaan Serbuk Infus Bekatul Sebagai Bahan Baku Bekatul Dextrosa Agar Untuk Pertumbuhan Jamur
Abstract
Rice bran is a waste of rice processing that can be used as a growth media of fungi because it contains high carbohydrates and high protein. Rice bran infusion is made in powder form using freeze-drying techniques to maintain the nutritional content of rice bran. This study aims to determine the level of carbohydrate and protein media made from bran infusion powder and determine the dose of bran infusion powder in the preparation of dextrose bran media. In this study using the Luff Schoorl method to measure the levels of carbohydrate media and the Kjeldhal method to measure the protein content of bran media. Candida albicans, Mallasezia furfur, Aspergillus fumigatus were inoculated in each medium. From this study, the highest levels of carbohydrate (9.11%) and protein (1.64%) were obtained in media using 200 g of rice bran infusion (iBDA200) and the lowest levels carbohydrate (0.44%) and protein (0.08%) wer obtained in media using 2 g of rice bran infusion powder (SBDA2). Growth of M. furfur, C. albicans and A. fumigatus the most fertile on iBDA200 media. This study shows that carbohydrate and protein content are higher in media made from bran infusion. 10 g of rice bran infusion powder (SDA10) best grows fungi but has no significant correlation with fungi growth.
References
Aini, N., 2015. Media Alternatif untuk Pertumbuhan Jamur Menggunakan Sumber Karbohidrat yang Berbeda. Universitas Muhammadiyah Surakarta.
Askew, C., Sellam, A., Epp, E., Hogues, H., Mullick, A., Nantel, A., and Whiteway, M., 2009. Transcriptional regulation of carbohydrate metabolism in the human pathogen Candida albicans. PLoS Pathogens. 5(10).
Banerjee, A., Ganesan, K., and Datta, A., 1991. Induction of secretory acid proteinase in Candida albicans. Journal of General Microbiology. 137(10): 2455–2461.
Basarang, M., Naim, N., dan Rahmawati. 2018. Perbandingan Pertumbuhan Jamur pada Media Bekatul Dextrose Agar (BDA) dan Potato Dextrose Agar (PDA). Seminar Nasional Hasil Penelitian. (November): 121–125.
Basarang, M., dan Rianto, M. R., 2018. Pertumbuhan Candida sp dan Aspergillus sp dari Bilasan Bronkus Penderita Tuberkulosis Paru pada Media Bekatul. 9 (18), 74–82.
Basarang, M., Rianto, M. R., dan Magfirah. 2016. Penambahan Glukosa pada Media Bekatul Agar untuk Pertumbuhan Aspergillus sp. Jurnal Medika: Media Ilmiah Analis Keehatan. 1(2): 56–61.
Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., and Khurana, S., 2015. Evolution of bacterial and fungal growth media. Bioinformation. 11(4): 182–184.
Bhosale, S., and Vijayalakshmi, D., 2015. Processing and nutritional composition of rice bran. Current Research in Nutrition and Food Science, 3(1), 74–80.
Cappucino, J. G., and Sherman, N., 2014. Manual Laboratorium Mikrobiologi. 8th ed. J. Manurung dan H. Vidhayanti, Ed.. Jakarta: EGC.
Dahlan, M. S., 2001. Statistik untuk Kedoktera Kesehatan. 4th ed. Jakarta: Salemba Medika.
Dewi, C., Purwoko, T., dan Pangastuti, A., 2005. Produksi Gula Reduksi oleh Rhizopus oryzae dari Substrat Bekatul. Bioteknologi. 2(1), 21–26.
Ene, I. V., Brunke, S., Brown, A. J. P., and Hube, B., 2014. Metabolism in fungal pathogenesis. Cold Spring Harbor Perspectives in Medicine, 4(12): 1–21.
Food Drug and Administration. 2017. BAM Media M127: Potato Dextrose Agar.
Gupta, M., Manisha, K., and Grover, R., 2012. Effect of Various Media Types on the Rate of Growth of Aspergillus niger. Indian Journal of Fundamental and Applied Life Sciences. 2(2): 141–144.
Kwoseh, C. K.., Asomani-Darko, M., and Adubofour, K., 2012. Cassava starch-agar blend as alternative gelling agent for mycological culture media. Bots. J. Agric. Appl. Sci, 8(1).
Labconco. 2010. A Guide To Freeze Drying for the Laboratory. Industry Service Publication. 11.
Martiansyah, I., dan Putranto, R. A., 2017. Pemanfaatan teknologi liofilisasi ( freeze drying ) dalam pengawetan sampel.
Morschhäuser, J., 2011. Nitrogen regulation of morphogenesis and protease secretion in Candida albicans. International Journal of Medical Microbiology.
Naim, N., 2016. Pemanfaatan Bekatul Sebagai Media Alternatif untuk Pertumbuhan Aspergillus sp. Media Analis Kesehatan. 2(2): 1–6.
Pujihastuti, I. (2009). Teknologi Pengawetan Buah Tomat Dengan Metode Freeze Drying. Metana - Media Komunikasi Rekayasa Proses Dan Teknologi Tepat Guna, 6(01). https://doi.org/10.14710/metana.v6i01.1948
Ramachandra, S., Linde, J., Brock, M., Guthke, R., Hube, B., & Brunke, S. (2014). Regulatory networks controlling nitrogen sensing and uptake in Candida albicans. PLoS ONE, 9(3). https://doi.org/10.1371/journal.pone.0092734
Ravimannan, N., Arulanantham, R., Pathmanathan, S., & Niranjan, K. (2014). Alternative culture media for fungal growth using different formulation of plant material. International Journal of Pharma and Bio Science, 5(1), 36–39. https://doi.org/10.22376/ijpbs.2017.8.1.b445-452
Sharma, G., and Pandey, R. R., 2010. Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes. Journal of Yeast and Fungal Research. 1(8): 157–164.
Standar Nasional Indonesia. 1992. Cara Uji Makanan dan Minuman. Pub. L. No. 01-2891–1992.
Uthayasooriyan, M., Pathmanathan, S., Ravimannan, N., and Sathyaruban, S., 2016. Formulation of alternative culture media for bacterial and fungal growth. Der Pharmacia Lettre. 8(1): 444–449.
Askew, C., Sellam, A., Epp, E., Hogues, H., Mullick, A., Nantel, A., and Whiteway, M., 2009. Transcriptional regulation of carbohydrate metabolism in the human pathogen Candida albicans. PLoS Pathogens. 5(10).
Banerjee, A., Ganesan, K., and Datta, A., 1991. Induction of secretory acid proteinase in Candida albicans. Journal of General Microbiology. 137(10): 2455–2461.
Basarang, M., Naim, N., dan Rahmawati. 2018. Perbandingan Pertumbuhan Jamur pada Media Bekatul Dextrose Agar (BDA) dan Potato Dextrose Agar (PDA). Seminar Nasional Hasil Penelitian. (November): 121–125.
Basarang, M., dan Rianto, M. R., 2018. Pertumbuhan Candida sp dan Aspergillus sp dari Bilasan Bronkus Penderita Tuberkulosis Paru pada Media Bekatul. 9 (18), 74–82.
Basarang, M., Rianto, M. R., dan Magfirah. 2016. Penambahan Glukosa pada Media Bekatul Agar untuk Pertumbuhan Aspergillus sp. Jurnal Medika: Media Ilmiah Analis Keehatan. 1(2): 56–61.
Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., and Khurana, S., 2015. Evolution of bacterial and fungal growth media. Bioinformation. 11(4): 182–184.
Bhosale, S., and Vijayalakshmi, D., 2015. Processing and nutritional composition of rice bran. Current Research in Nutrition and Food Science, 3(1), 74–80.
Cappucino, J. G., and Sherman, N., 2014. Manual Laboratorium Mikrobiologi. 8th ed. J. Manurung dan H. Vidhayanti, Ed.. Jakarta: EGC.
Dahlan, M. S., 2001. Statistik untuk Kedoktera Kesehatan. 4th ed. Jakarta: Salemba Medika.
Dewi, C., Purwoko, T., dan Pangastuti, A., 2005. Produksi Gula Reduksi oleh Rhizopus oryzae dari Substrat Bekatul. Bioteknologi. 2(1), 21–26.
Ene, I. V., Brunke, S., Brown, A. J. P., and Hube, B., 2014. Metabolism in fungal pathogenesis. Cold Spring Harbor Perspectives in Medicine, 4(12): 1–21.
Food Drug and Administration. 2017. BAM Media M127: Potato Dextrose Agar.
Gupta, M., Manisha, K., and Grover, R., 2012. Effect of Various Media Types on the Rate of Growth of Aspergillus niger. Indian Journal of Fundamental and Applied Life Sciences. 2(2): 141–144.
Kwoseh, C. K.., Asomani-Darko, M., and Adubofour, K., 2012. Cassava starch-agar blend as alternative gelling agent for mycological culture media. Bots. J. Agric. Appl. Sci, 8(1).
Labconco. 2010. A Guide To Freeze Drying for the Laboratory. Industry Service Publication. 11.
Martiansyah, I., dan Putranto, R. A., 2017. Pemanfaatan teknologi liofilisasi ( freeze drying ) dalam pengawetan sampel.
Morschhäuser, J., 2011. Nitrogen regulation of morphogenesis and protease secretion in Candida albicans. International Journal of Medical Microbiology.
Naim, N., 2016. Pemanfaatan Bekatul Sebagai Media Alternatif untuk Pertumbuhan Aspergillus sp. Media Analis Kesehatan. 2(2): 1–6.
Pujihastuti, I. (2009). Teknologi Pengawetan Buah Tomat Dengan Metode Freeze Drying. Metana - Media Komunikasi Rekayasa Proses Dan Teknologi Tepat Guna, 6(01). https://doi.org/10.14710/metana.v6i01.1948
Ramachandra, S., Linde, J., Brock, M., Guthke, R., Hube, B., & Brunke, S. (2014). Regulatory networks controlling nitrogen sensing and uptake in Candida albicans. PLoS ONE, 9(3). https://doi.org/10.1371/journal.pone.0092734
Ravimannan, N., Arulanantham, R., Pathmanathan, S., & Niranjan, K. (2014). Alternative culture media for fungal growth using different formulation of plant material. International Journal of Pharma and Bio Science, 5(1), 36–39. https://doi.org/10.22376/ijpbs.2017.8.1.b445-452
Sharma, G., and Pandey, R. R., 2010. Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes. Journal of Yeast and Fungal Research. 1(8): 157–164.
Standar Nasional Indonesia. 1992. Cara Uji Makanan dan Minuman. Pub. L. No. 01-2891–1992.
Uthayasooriyan, M., Pathmanathan, S., Ravimannan, N., and Sathyaruban, S., 2016. Formulation of alternative culture media for bacterial and fungal growth. Der Pharmacia Lettre. 8(1): 444–449.
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