Analisis Resiko Kejadian Akut Rekuren Demam Tifoid dan Hubungannya dengan Kadar Protein Nucleotide Binding Oligomerization Domain 2 (NOD2)

Abstract

Typhoid fever is an infectious disease caused by Salmonella typhi bacteria and is endemic. The NOD2 gene is one of the host susceptibility genes in people with typhoid fever. NOD2 acts as an intracellular receptor that binds to the muramyl dipeptide ligand derived from bacterial peptidoglycan. The purpose of this study was to determine the levels of NOD2 protein in Acute Recurrent of Typhoid Fever (ARTF), typhoid fever (TF) patients, and healthy people (HP). Enzyme-linked Immunosorbent Assay (ELISA) was used to analyze NOD2 levels. The data analysis used was the student t-test. A significant difference of NOD2 level was found between the ARTF and TF group compared HP group (p<0.001). Then there is a negative correlation between NOD2 levels and widal titer with R2 coefficient of -0.262 and p value = 0.013. There was a decrease in NOD2 protein expression in patients with TF and ARTF, so it can be concluded that the lower the NOD2 levels, the higher the likelihood of TF and ARTF. Then the negative correlation between NOD2 and widal titer indicates that the higher the widal titer, the lower the NOD2 level results.