Uji Antibakteri Isolat Endofit Asal Tanaman Kayu Jawa Lannea coromandelica (Houtt.) Merr. Terhadap MRSA Methicilin-Resistant Staphylococcus aureus
Abstract
Asia is one of the regions with the highest incidence of Methicillin-resistant Staphylococcus aureus (MRSA) in the world. Endophytes in the tropics have not been widely studied and have not been explored, especially those related to their potential uses in medicine, agriculture, and other industries. Several research reports have also found that endophytic bacteria provide great potential in producing a variety of new natural products, including secondary metabolites that are similar to its host. This research is a true experimental laboratory to analyze the inhibition of endophytic bacterial isolates from kayu jawa (Lanneacoromandelica (Houtt) Merr on MRSA. The inhibitory test in this study uses the disc diffusion method. Antibacterial results The experimental results of the inhibitory test are expressed as the average ± standard deviation (SD) of two replications and the data is processed using Microsoft Excel 2007. Twelve endophytic bacterial isolates from kayu jawa have the ability to inhibit MRSA growth. visible from the formation of a clear zone around the paper disk.Based on the results of the measurement of the area of the clear zone formed, the twelve endophytic bacterial isolates are included in the strong category (11-20 mm) in inhibiting the growth of pathogens and of the twelve endophytic bacterial isolates, KJA3 isolates and KJB1 isolate which have ability inhibited the growth of MRSA best compared to other isolates because it has the largest inhibitory zone area of 17 mm and 16 mm.
References
Abdalla M. A., and Matasyoh J. C., 2014. Endophytes as Producers of Peptides: an Overview bout the Recently Discovered Peptides from Endophytic Microbes. Nat. Prod. Bioprospect. 4(5): 257–270.
Chen, C. J., Lin, M. H., Shu J. C., Lu, J. J., 2014. Reduced Susceptibility to Vancomycin in Isogenic Staphylococcus aureus Strains of Sequence Type 59: Tracking Evolution and Identifying Mutations by Whole-Genome Sequencing. J Antimicrob Chemother. 69: 349–354.
Chuang Y. Y, and Huang Y. C., 2013. Molecular Epidemiology of Community-Associated Meticillin-Resistant Staphylococcus aureus in Asia. Lancet Infect Dis. 13: 698–708.
Castillo, U. F., Strobel, G. A., Mullenberg, K., Condron, M. M., Teplow, D. B., Folgiano, V., Gallo, M., Ferracane, R., Mannina, L., Viel, S., Codde, M., Robison, R., Porter, H. and Jensen, J., 2006. Munumbicins E-4 and E-5: Novel Broad-Spectrum Antibiotics from Streptomyces NRRL 3052. FEMS Microbiology Letter. 255: 296-300.
Ding, L., Armin, M., Heinz-Herbert, F., Wen-Han, L. and Christian, H., 2011. A Family of Multi-Cyclic Indolo Sesquiterpenes from A Bacterial Endophyte. Organic and Biomolecular Chemistry. 9: 4029-4031.
Dancer, S. J., Robb, A., Crawford, A. and Morrison, D., 2003. Oral Streptogramins in the Management of Patients with Methicillin-Resistant Staphylococcus aureus (MRSA) Infections. Journal of Antimicrobial Chemotherapy. 51: 731-735.
Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H, Rai M., 2015. Endophytic Actinobacteria of Medicinal Plants: Diversity and Bioactivity. Anton Van Leeuwenhoek. 108(2): 267–289.
Grundmann H, Aires de Sousa M, Boyce J, Tiemersma E., 2006. Emergence and Resurgence of Methicillin-Resistant Staphylococcus aureus as A Public-Health Threat. Lancet. 368: 874–885.
Handayani D., Sandrawaty N., Murniati M., Regina R., 2015. Screening of Endophytic Bacteria Isolated from Marine Sponge Haliclona fascigera for Inhibition Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus (MRSA). Journal of Applied Pharmaceutical Science. 5(09) : 139-142.
Kauffman, C. A., 2003. Therapeutic and Preventative Options for The Management of Vancomycin-Resistant Enterococcal Infections. Journal of Antimicrobial Chemotherapy. 51: 23-30.
Lee, D.R., Cheng, J., Lee, S.K., Hong, H.J., Song, J., Yang, S.H., Suh, J.W., 2016. Anti-Multi Drug Resistant Pathogen Activity of Siderochelin A, Produced by A Novel Amycolatopsis sp. KCTC 29142. Kor. J. Microbiol. 52(3).
Machavariani N. G., Ivankova T.D., Sineva O.N., Terekhova L.P., 2014. Isolation of Endophytic Actinomycetes from Medicinal Plants of the Moscow region, Russia, World Appl. Sci. J. 30(11): 1599–1604.
Paramudita A. E., Ramdani, Dini I., 2017. Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Kulit Batang Kayu Jawa Lannea coromandelica (Houtt) Merr. Jurnal Chemica. 18: 64-75.
Prawirodiharjo, Erwin. 2014. Uji Aktivitas Antioksidan dan Uji Toksisitas Ekstrak Etanol 70% dan Ekstrak Air Kulit Batang Kayu Jawa (Lannea coromandelica). Skripsi UIN Syarif Hidayatullah.
Rahmadani, Fitri. 2015. Uji Aktivitas Antibakteri dari Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, dan Pseudomonas aeruginosa. Skripsi UIN Syarif Hidayatullah.
Rao, V.S., Eintein, J.W., Das, K., 2014. Hepatoprotecctive and Antioxidant Activity of Lannea coromandelica Linn. on Thioacetamide Induced Hepatotoxicity in Rats. International Letters Of Natural Science J. 3: 30-43.
Sanchez, S., Demain, A. L., 2014. Valuable Products from Microbes, in: G. Neelam, A. Abhinav (Eds.), Microbes in Process, USA: Nova Scientific Publishers Inc. pp. 23–57.
Tonial, F., Maia, B.H., Gomes-Figueiredo, J.A., Sobottka, A.M., Bertol, C.D., Nepel, A., 2016. Influence of Culturing Conditions on Bioprospecting and the Antimicrobial Potential of Endophytic Fungi from Schinus terebinthifoliu. Curr. Microbiol. 72(2):173–183.
Tanvir, R., Sajid, I., Hasnain, S., Kulik, A., Grond, S., 2016. Rare Actinomycetes Nocardia Caishijiensis and Pseudonocardia Carboxydivorans as Endophytes, Their Bioactivity and Metabolites Evaluation. Microbiol. Res. 185: 22–35.
Van Hal, S.J., Lodise, T.P., Paterson D.L., 2012. The Clinical Significance of Vancomycin Minimum Inhibitory Concentration in Staphylococcus aureus Infections: A Systematic Review and Meta-Analysis. Clin Infect Dis. 54: 755–771
Chen, C. J., Lin, M. H., Shu J. C., Lu, J. J., 2014. Reduced Susceptibility to Vancomycin in Isogenic Staphylococcus aureus Strains of Sequence Type 59: Tracking Evolution and Identifying Mutations by Whole-Genome Sequencing. J Antimicrob Chemother. 69: 349–354.
Chuang Y. Y, and Huang Y. C., 2013. Molecular Epidemiology of Community-Associated Meticillin-Resistant Staphylococcus aureus in Asia. Lancet Infect Dis. 13: 698–708.
Castillo, U. F., Strobel, G. A., Mullenberg, K., Condron, M. M., Teplow, D. B., Folgiano, V., Gallo, M., Ferracane, R., Mannina, L., Viel, S., Codde, M., Robison, R., Porter, H. and Jensen, J., 2006. Munumbicins E-4 and E-5: Novel Broad-Spectrum Antibiotics from Streptomyces NRRL 3052. FEMS Microbiology Letter. 255: 296-300.
Ding, L., Armin, M., Heinz-Herbert, F., Wen-Han, L. and Christian, H., 2011. A Family of Multi-Cyclic Indolo Sesquiterpenes from A Bacterial Endophyte. Organic and Biomolecular Chemistry. 9: 4029-4031.
Dancer, S. J., Robb, A., Crawford, A. and Morrison, D., 2003. Oral Streptogramins in the Management of Patients with Methicillin-Resistant Staphylococcus aureus (MRSA) Infections. Journal of Antimicrobial Chemotherapy. 51: 731-735.
Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H, Rai M., 2015. Endophytic Actinobacteria of Medicinal Plants: Diversity and Bioactivity. Anton Van Leeuwenhoek. 108(2): 267–289.
Grundmann H, Aires de Sousa M, Boyce J, Tiemersma E., 2006. Emergence and Resurgence of Methicillin-Resistant Staphylococcus aureus as A Public-Health Threat. Lancet. 368: 874–885.
Handayani D., Sandrawaty N., Murniati M., Regina R., 2015. Screening of Endophytic Bacteria Isolated from Marine Sponge Haliclona fascigera for Inhibition Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus (MRSA). Journal of Applied Pharmaceutical Science. 5(09) : 139-142.
Kauffman, C. A., 2003. Therapeutic and Preventative Options for The Management of Vancomycin-Resistant Enterococcal Infections. Journal of Antimicrobial Chemotherapy. 51: 23-30.
Lee, D.R., Cheng, J., Lee, S.K., Hong, H.J., Song, J., Yang, S.H., Suh, J.W., 2016. Anti-Multi Drug Resistant Pathogen Activity of Siderochelin A, Produced by A Novel Amycolatopsis sp. KCTC 29142. Kor. J. Microbiol. 52(3).
Machavariani N. G., Ivankova T.D., Sineva O.N., Terekhova L.P., 2014. Isolation of Endophytic Actinomycetes from Medicinal Plants of the Moscow region, Russia, World Appl. Sci. J. 30(11): 1599–1604.
Paramudita A. E., Ramdani, Dini I., 2017. Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Kulit Batang Kayu Jawa Lannea coromandelica (Houtt) Merr. Jurnal Chemica. 18: 64-75.
Prawirodiharjo, Erwin. 2014. Uji Aktivitas Antioksidan dan Uji Toksisitas Ekstrak Etanol 70% dan Ekstrak Air Kulit Batang Kayu Jawa (Lannea coromandelica). Skripsi UIN Syarif Hidayatullah.
Rahmadani, Fitri. 2015. Uji Aktivitas Antibakteri dari Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, dan Pseudomonas aeruginosa. Skripsi UIN Syarif Hidayatullah.
Rao, V.S., Eintein, J.W., Das, K., 2014. Hepatoprotecctive and Antioxidant Activity of Lannea coromandelica Linn. on Thioacetamide Induced Hepatotoxicity in Rats. International Letters Of Natural Science J. 3: 30-43.
Sanchez, S., Demain, A. L., 2014. Valuable Products from Microbes, in: G. Neelam, A. Abhinav (Eds.), Microbes in Process, USA: Nova Scientific Publishers Inc. pp. 23–57.
Tonial, F., Maia, B.H., Gomes-Figueiredo, J.A., Sobottka, A.M., Bertol, C.D., Nepel, A., 2016. Influence of Culturing Conditions on Bioprospecting and the Antimicrobial Potential of Endophytic Fungi from Schinus terebinthifoliu. Curr. Microbiol. 72(2):173–183.
Tanvir, R., Sajid, I., Hasnain, S., Kulik, A., Grond, S., 2016. Rare Actinomycetes Nocardia Caishijiensis and Pseudonocardia Carboxydivorans as Endophytes, Their Bioactivity and Metabolites Evaluation. Microbiol. Res. 185: 22–35.
Van Hal, S.J., Lodise, T.P., Paterson D.L., 2012. The Clinical Significance of Vancomycin Minimum Inhibitory Concentration in Staphylococcus aureus Infections: A Systematic Review and Meta-Analysis. Clin Infect Dis. 54: 755–771
Authors
Copyright and license info is not available