Molecular Identification of Cellulolytic Bacteria  From Mangrove Sediment at Tin Minning Region In West Bangka

Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; andi kurniawan; abu bakar sambah; asep awaludin prihanto

doi:10.20956/ijab.v3i1.5848

Issue

Vol. 3 No. 1 (2019): International Journal of Applied Biology

Issue Published : Jun 29, 2019

Date Log

Submitted

Jan 4, 2019

Published

Jun 29, 2019

Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka

https://doi.org/10.20956/ijab.v3i1.5848

Ardiansyah Kurniawan

Bangka Belitung University

Suci Puspita Sari

Bangka Belitung University

Euis Asriani

Bangka Belitung University

andi kurniawan

Brawijaya University

abu bakar sambah

brawijaya university

asep awaludin prihanto

Brawijaya university

Corresponding Author(s) : Ardiansyah Kurniawan

ardian_turen@yahoo.co.id

International Journal of Applied Biology, Vol. 3 No. 1 (2019): International Journal of Applied Biology
Article Published : Jun 29, 2019

Abstract

Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.

References

Andriani, Y., Sastrawibawa, S., Safitri, R. & Abun. 2012. Isolation and Identification of Cellulolytic Microbes as Biodegradators Rough Fiber in Feed Materials from Agricultural Waste. IJAS. 2 (3).
Arta, A.P, Maidie, A. & Saptiani, G. 2009. The Effect Of Mangrove Vegetation Treatment On Vibrio Sp Bacterial Population In Bontang Coast. Jurnal Kehutanan Tropika Humida. 2 (2): 133-142.
Behera, B.C., Sethi, B.K., Mishra, R.R., Dutta, S.K. & Thatoi, H.N. 2017. Microbial cellulases – Diversity & biotechnology with reference to mangrove environment: A review. Journal of Genetic Engineering and Biotechnology. 15 : 197–210.
Chantarasiri, A. 2015. Aquatic Bacillus cereus JD0404 isolated from the muddy sediments of mangrove swamps in Thailand and characterization of its cellulolytic activity. Egypt J Aquat Res. 41(3):257–64.
Gao, Z.M., Xiao, J., Wang, X.N., Ruan, L.W., Chen, X.L. & Zhang, Y.Z. 2011. Int. J. Syst. Evol. Microbiol. 62: 1958–1962.
Geetha, I., Manonmani, A.M. & Prabakaran, G. 2011. Bacillus amyloliquefaciens: A mosquitocidal bacterium from mangrove forests of Andaman & Nicobar islands, India. Acta Tropica. 120. (3): 155-159.
Hapsoh, Wawan, Dini, I.R. & Siregar, J.A.. 2017. Compatibility Tests of Potential Cellulolytic Bacteria and Growth Optimization in Several Organic Materials. International Journal of Science and Applied Technology. 2 (2).
Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018b. Mangrove Leaf Litter Cellulolytic Bacteria on Bangka Island. Samakia. 9 (1).
Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018c. Isolation and Identification of Cellulose Degradation Bacteria from Tukak Sadai Mangrove Ecosystem, South Bangka. Jurnal Perikanan Pantura. Vol. 1. No.2
Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018d. Cellulolytic Bacteria in Weathered Wood in Sungailiat Mangrove, Bangka Dan Tukak Sadai, South Bangka. Proceedings of the National Wetland Environment Seminar. 3 (1): 301-305.
Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018a. Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island. IOP Conf. Series: Earth and Environmental Science 137.
Kurniawan, A. 2012. Introduction To Post-Tin Mining Aquaculture. UBB Press.
Kurniawan. A, and N. Ekowati, 2016. Mycoremediation of Heavy Metal: A Review. Bioteknologi & Biosains Indonesia. 3 (1) : 36-45.
Letchumanan. V, Chan, K.G. & Lee, L.H. 2014. Vibrio parahaemolyticus: A Review on the Pathogenesis, Prevalence and Advance Molecular Identification Techniques. Frontiers in microbiology. 5 (705)
Maiti, S.K & Chowdhury, A. 2013. Effects of Anthropogenic Pollution on Mangrove Biodiversity : A Review. Journal of Environmental Protection. 4 : 1428-1434.
Ningsih, R.L., Khotimah, S. & Lovadi, I. 2014. Cellulose Degradation Bacteria from Avicennia alba Blume Leaf Litter in the Safety Mangrove Forest in Pontianak Regency. Protobiont. 3 (1): 34 – 40
Prihanto, A.A, & Wakayama, M. 2016. Adv. Food Nutr. Res. 79 1–25.
Rigonato, J. 2017. Temporal assessment of microbial communities in soils of two contrasting mangroves. Braz J Microbiol. http://dx.doi.org/10.1016/j.bjm.2017.04.008
Sari, S. P. & Rosalina, D. 2014. Success Rate of Mangrove Planting in Post-Mining Land in South Bangka Regency. Maspari Journal. 6 (2).
Shobharani, P., Yogesh, D., Halami. P.M. & Sachindra. N.M. 2013. Potential of Cellulase From Bacillus megaterium for Hydrolysis of Sargassum. Journal of Aquatic Food Product Technology. 22 (5). 520-535.
Soeka,Y.S., Rahmansyah, M. & Sulistiani. 2015. Optimization of α-Amylase Enzyme from Bacillus amyloliquefaciens O1 which are Induced by Rice Bran and Carboxymethylcellulose. Jurnal Biologi. 11 (2) : 259-266.
Susanto. 2015. Tin-Bottom Areas in Bangka Belitung with Spot_6 Satellite Data. National Seminar on Science and Technology 2015. Faculty of Engineering, Muhammadiyah University, Jakarta.
Umroh. 2015. Seeding and Planting Rhizophora apiculata in post-conventional tin mining (TI) areas in Muara Kudai, Bangka Regency.Jurnal Kelautan. 8 (1).
Yahya, Happy, N., Yenny, R. & Soemarno. 2014. Characteristic of extracelluler metabolic of coastal ecosystem bacteria producing histidine decarboxylase crude. International Food Research Journal. 21 (4) : 1337-1347
Yulma, Ihsan, B., Sunarti, Malasari, E., Wahyuni, N. & Mursyban. 2017. Identification of Bacteria in Decomposed Mangrove Leaf Litter in Mangrove and Proboscis Conservation Areas in Tarakan City. Journal of Tropical Biodiversity and Biotechnology. 2. 28-33.

References

Andriani, Y., Sastrawibawa, S., Safitri, R. & Abun. 2012. Isolation and Identification of Cellulolytic Microbes as Biodegradators Rough Fiber in Feed Materials from Agricultural Waste. IJAS. 2 (3).

Arta, A.P, Maidie, A. & Saptiani, G. 2009. The Effect Of Mangrove Vegetation Treatment On Vibrio Sp Bacterial Population In Bontang Coast. Jurnal Kehutanan Tropika Humida. 2 (2): 133-142.

Behera, B.C., Sethi, B.K., Mishra, R.R., Dutta, S.K. & Thatoi, H.N. 2017. Microbial cellulases – Diversity & biotechnology with reference to mangrove environment: A review. Journal of Genetic Engineering and Biotechnology. 15 : 197–210.

Chantarasiri, A. 2015. Aquatic Bacillus cereus JD0404 isolated from the muddy sediments of mangrove swamps in Thailand and characterization of its cellulolytic activity. Egypt J Aquat Res. 41(3):257–64.

Gao, Z.M., Xiao, J., Wang, X.N., Ruan, L.W., Chen, X.L. & Zhang, Y.Z. 2011. Int. J. Syst. Evol. Microbiol. 62: 1958–1962.

Geetha, I., Manonmani, A.M. & Prabakaran, G. 2011. Bacillus amyloliquefaciens: A mosquitocidal bacterium from mangrove forests of Andaman & Nicobar islands, India. Acta Tropica. 120. (3): 155-159.

Hapsoh, Wawan, Dini, I.R. & Siregar, J.A.. 2017. Compatibility Tests of Potential Cellulolytic Bacteria and Growth Optimization in Several Organic Materials. International Journal of Science and Applied Technology. 2 (2).

Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018b. Mangrove Leaf Litter Cellulolytic Bacteria on Bangka Island. Samakia. 9 (1).

Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018c. Isolation and Identification of Cellulose Degradation Bacteria from Tukak Sadai Mangrove Ecosystem, South Bangka. Jurnal Perikanan Pantura. Vol. 1. No.2

Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018d. Cellulolytic Bacteria in Weathered Wood in Sungailiat Mangrove, Bangka Dan Tukak Sadai, South Bangka. Proceedings of the National Wetland Environment Seminar. 3 (1): 301-305.

Kurniawan, A., Sari, S.P., Asriani, E., Kurniawan, A., Sambah, A.B. & Prihanto, A.W. 2018a. Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island. IOP Conf. Series: Earth and Environmental Science 137.

Kurniawan, A. 2012. Introduction To Post-Tin Mining Aquaculture. UBB Press.

Kurniawan. A, and N. Ekowati, 2016. Mycoremediation of Heavy Metal: A Review. Bioteknologi & Biosains Indonesia. 3 (1) : 36-45.

Letchumanan. V, Chan, K.G. & Lee, L.H. 2014. Vibrio parahaemolyticus: A Review on the Pathogenesis, Prevalence and Advance Molecular Identification Techniques. Frontiers in microbiology. 5 (705)

Maiti, S.K & Chowdhury, A. 2013. Effects of Anthropogenic Pollution on Mangrove Biodiversity : A Review. Journal of Environmental Protection. 4 : 1428-1434.

Ningsih, R.L., Khotimah, S. & Lovadi, I. 2014. Cellulose Degradation Bacteria from Avicennia alba Blume Leaf Litter in the Safety Mangrove Forest in Pontianak Regency. Protobiont. 3 (1): 34 – 40

Prihanto, A.A, & Wakayama, M. 2016. Adv. Food Nutr. Res. 79 1–25.

Rigonato, J. 2017. Temporal assessment of microbial communities in soils of two contrasting mangroves. Braz J Microbiol. http://dx.doi.org/10.1016/j.bjm.2017.04.008

Sari, S. P. & Rosalina, D. 2014. Success Rate of Mangrove Planting in Post-Mining Land in South Bangka Regency. Maspari Journal. 6 (2).

Shobharani, P., Yogesh, D., Halami. P.M. & Sachindra. N.M. 2013. Potential of Cellulase From Bacillus megaterium for Hydrolysis of Sargassum. Journal of Aquatic Food Product Technology. 22 (5). 520-535.

Soeka,Y.S., Rahmansyah, M. & Sulistiani. 2015. Optimization of α-Amylase Enzyme from Bacillus amyloliquefaciens O1 which are Induced by Rice Bran and Carboxymethylcellulose. Jurnal Biologi. 11 (2) : 259-266.

Susanto. 2015. Tin-Bottom Areas in Bangka Belitung with Spot_6 Satellite Data. National Seminar on Science and Technology 2015. Faculty of Engineering, Muhammadiyah University, Jakarta.

Umroh. 2015. Seeding and Planting Rhizophora apiculata in post-conventional tin mining (TI) areas in Muara Kudai, Bangka Regency.Jurnal Kelautan. 8 (1).

Yahya, Happy, N., Yenny, R. & Soemarno. 2014. Characteristic of extracelluler metabolic of coastal ecosystem bacteria producing histidine decarboxylase crude. International Food Research Journal. 21 (4) : 1337-1347

Yulma, Ihsan, B., Sunarti, Malasari, E., Wahyuni, N. & Mursyban. 2017. Identification of Bacteria in Decomposed Mangrove Leaf Litter in Mangrove and Proboscis Conservation Areas in Tarakan City. Journal of Tropical Biodiversity and Biotechnology. 2. 28-33.

Issue

Vol. 3 No. 1 (2019): International Journal of Applied Biology

Date Log

Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka

Corresponding Author(s) : Ardiansyah Kurniawan

Abstract

References

Downloads