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Vol. 1 No. 1 (2017): International Journal of Applied Biology

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Submitted
Aug 10, 2017
Published
Aug 18, 2017

A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System

https://doi.org/10.20956/ijab.v1i1.2269
Sabrina Resky Pratiwi
Hasanuddin University
Nur Ainiah
Hasanuddin University
Hardyanti Hardyanti
Hasanuddin University
Dini Rusdayanti Putri
Hasanuddin University
Emilia Utomo
Hasanuddin University

Corresponding Author(s) : Sabrina Resky Pratiwi

sabrinareskyp7@gmail.com

International Journal of Applied Biology, Vol. 1 No. 1 (2017): International Journal of Applied Biology

Abstract

IntroductionEarthworms (Lumbricus rubellus) extract is known to contain  bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome. MethodsEarthworms powder was macerated using 50% ethanol for 3 days. The extract was  formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible.ResultsThe results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol.ConclusionsThe highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%.Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.  
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Author Biographies

Sabrina Resky Pratiwi, Hasanuddin University
Faculty of Pharmac
Nur Ainiah, Hasanuddin University
Faculty of Pharmacy
Hardyanti Hardyanti, Hasanuddin University
Faculty of Pharmacy
Dini Rusdayanti Putri, Hasanuddin University
Faculty of Pharmacy
Emilia Utomo, Hasanuddin University
Faculy of Pharmacy
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