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Vol. 5 No. (1) (2021): International Journal of Applied Biology

Date Log

Submitted
Jul 18, 2020
Published
Jul 6, 2021

Molecular identification and antibiotic susceptibility patterns of bacterial isolates from urine samples of African buffalo, eland and cattle

https://doi.org/10.20956/ijab.v5i(1).10748
Harry Asena Musonye
Kenyatta University
http://orcid.org/0000-0002-8372-7322
Ezekiel Mugendi Njeru
Kenyatta University
Ahmed Hassanali
Kenyatta University
Lydia Mali Langata
Kenyatta University
James Nonoh
Kenyatta University

Corresponding Author(s) : Harry Asena Musonye

musonyefifth90@gmail.com

International Journal of Applied Biology, Vol. 5 No. (1) (2021): International Journal of Applied Biology

Abstract

Presence of antibiotic resistant bacteria in natural environment is an escalating risk of serious implication on human and animal health. Livestock and wildlife have been long recognized as reservoirs for antibiotic resistant bacteria. Nonetheless, there is limited knowledge regarding the potential of livestock and wildlife urine to act as transmission corridor for the spread of antibiotic resistant bacteria. The present study aimed at evaluating antibiotic susceptibility patterns and molecular identification of bacteria isolated from livestock and wildlife urine samples. A total of 19 different bacteria isolated from urine samples of African buffalo, eland and cattle were subjected to antibiotic susceptibility test. The isolates showed diverse susceptibility patterns against co-trimoxazole, tetracycline, amoxycillin, ciprofloxacin, streptomycin, nalidixic acid, chloramphenicol and gentamicin. Of the tested isolates, 73.7 % were exhibited resistance while 31.6 % were intermediate to the range of antibiotics tested. High resistance prevalence to amoxicillin (58%), tetracycline (26 %) and co-trimoxazole (11%) by the tested bacteria was observed. This study reveals the bacteria associated with African buffalo, eland and cattle urine as potential candidates for antibiotic resistance. This information demonstrates the need for measures to be adopted to limit the presence of antibiotic resistant bacteria in wildlife and livestock reservoirs.
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Author Biographies

Harry Asena Musonye, Kenyatta University
Department of Biochemistry, Microbiology and Biotechnology
Ezekiel Mugendi Njeru, Kenyatta University
Department of Biochemistry, Microbiology and Biotechnology
Ahmed Hassanali, Kenyatta University
Department of Chemistry
Lydia Mali Langata, Kenyatta University
Department of Biochemistry, Microbiology and Biotechnology
James Nonoh, Kenyatta University
Department of Biochemistry, Microbiology and Biotechnology
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