Issue

Vol. 4 No. (2) (2020): International Journal of Applied Biology

Date Log

Submitted
Jul 16, 2020
Published
Jul 6, 2021

Microbiological Assessment of Roasted Dried Periwinkle (Tympanotonus Fuscatus) Sold in Yenagoa Bayelsa State

https://doi.org/10.20956/ijab.v4i(2).10724
Obhioze Augustine Akpoka
Igbinedion University
http://orcid.org/0000-0001-9190-9188

Corresponding Author(s) : Obhioze Augustine Akpoka

ausbones@gmail.com

International Journal of Applied Biology, Vol. 4 No. (2) (2020): International Journal of Applied Biology

Abstract

The microbial load of dried periwinkle(tympanotonus fuscatus) sold in Opolo market, Swali market and Kpansia market in Yenagoa were assessed and the microorganisms isolated were identified. Three samples were bought from Opolo market, Swali market and Kpansia market respectively were assessed. Total bacterial populations of the samples from Opolo market range from 37×10-5- 12×10-5 cfu/g, Swali range from 49×10-5 - 29×10-5 cfu/g, while Kpansia market range from 38×10-5- 18×10-5 cfu/g. For fungi population, Opolo market range from 6×10-5 - 1×10-5 cfu/g, Swali market range from 9×10-5- 3×10-5 cfu/g, while Kpansia market range from 7×10-5 - 2×10-5cfu/g. The Samonella/Shigella counts from Swali market range from 13×10-5- 2×10-5 cfu/g, kpansia market range from 12×10-5- 3×10-5 cfu/g, while Opolo market range from 11×10-5-1×10-5 cfu/g. The coliform counts from Swali market range from 30×10-5 - 12×10-5 cfu/g, Kpansia market range from 28×10-5 - 10×10-5  cfu/g, while Opolo market range from 25×10-5- 11×10-5 cfu/g. For fungi population, Opolo market range from 6×10-5- 1×10-5 cfu/g, Swali market range from 9×10-5-3×10-5 cfu/g, while Kpansia market range from 7×10-5 - 2×10-5 cfu/g.The bacterial isolates belonged to six genera identified as: Klebsiella, Salmonella, Escherichia coli, Shigella, Staphylococcus and Bacillus. The percentage (%) occurrences of the bacterial isolates were: Bacillus sp. (28.5%), Staphylococcus aureus (25%), Shigella sp. (21.4%), Escherichia coli (17.9%), Salmonella sp. (3.6%) and Klebsiella sp. (3.6%). Four (4) fungal isolates were obtained from the dried periwinkle samples and two belonged to the genus Aspergillus, while the other two isolates belong to the genera Penicillium and Mucor. The percentage (%) occurrence of the fungal isolates were: Mucor (41.6%), Aspergillus flavus (25%), Aspergillus niger (16.7%) and Penicillium (16.7%). There was no significant difference in total viable count between one market and another at 5% level of significance (p0.05). Also, there was no significant difference in total viable count between one seller and other sellers. The occurrence of Bacillus, Shigella, Staphylococcus, Klebsiella, Escherichia coli, Salmonella, Aspergillus, and Mucor species are pinpointing the high pathogenicity and health hazard in consuming the dried periwinkle. Due to the soaring demand of this sea food (periwinkle) and the health hazard associated with microorganisms isolated from them as revealed in this study, additional concentration should be paid to safety through proper storage and handling processes and it is important that periwinkle should be properly cooked before consumption. 
References
  1. Abolagba, O.J., Adekunle, A., Dede, A., Omoigui, G.O. (2011) Microbial Assessment of smoked fish (Clarias spp) in Benin metropolis, Edo State, Nigeria. Nigerian Journal of Agricultural Food Environmental. 7(3): 55 – 58.
  2. Adams, M.R and Moss, M.O. (1999). Microbiology of primary food commodities:In, Food Microbiology, 3rd Edn. The Royal Society of Chemistry, Cambridge, U.K.Pp 122.
  3. Adebayo-Tayo, B.C., Onilude, A., Patric, U.G. (2008). Microflora of smoke-dried fishes sold in Uyo Eastern Nigeria. World J. Agric. Sci. 4: 346 – 350.
  4. Adewuyi, A. P. and Adegoke, T. (2008). Expository study of periwinkle shell as coarse aggregates in concrete work. Journal of Applied science and Research. Page 4 (12).
  5. Alagoa, E.J. (1999). Ed. The land and people of Bayelsa State: Central Niger Delta. Onyoma Research publications Choba Port Harcourt. pp. 10 -17.
  6. Aminigo, E.R. and Okoro, J.C. (2002). Microflora of smoke-dried seafoods marketed in the Niger Delta; A study in Port Harcourt, Rivers State. Trans Nigerian Social Biological Conservation (Special Edition) 1 – 7.
  7. American Academy of Pediatrics (2012). Red Book: Report of the Committee on Infectious Diseases. Pickering LK, ed. 29th ed. Elk Grove Village, IL: American Academy of Pediatrics. pp 791-792.
  8. American Public Health Association (2009). Cholera and Other Vibriosis. In D. Heymann (Ed.), Control of Communicable Diseases Manual. (19th ed., pp. 120-134).
  9. Archibong, N. A., Ofem, E. O., Nna, V. U., Bisong, E. M. B., Johnson, J. T. and Eno, A. E. (2014). Changes in Haematological Parameters Following the Administration of Crude Extract from Tympanotonus fuscatus (Periwinkle) in Rats. Austrelia Journal of Basic & Applied Science 8(10): 586-591.
  10. Ariahu, C. C. and Ilori, M.O. (1992). Use of periwinkle as source of dietary protein: The Nutritional, Toxicalogical, processing and policy implication. Food Review International Journal, vol. 8 (2) page 223-233.
  11. Austin, B., Austin, D.A., Blanch, A. R., Cerda, M., Grimont, P.A.D., Jofre, J., Koblavi, S., Larsen, J. L., Pedersen, K. and Tiainen, T. (1997). A comparison of methods for the typing of fish-pathogenic Vibrio spp. System Applied Microbiology, 20, 89–101.
  12. Ayers, J.C., Mudt, J. and Sardine, W. E. (1980). Microbiology of foods. W.H Freeman San Francisco. pp. 441 – 473.
  13. Baltimore, M. D., Williams, J., Wilkins, M., Christiansen, L.N. and King, N.S. (1991). The microbial content of some salads and sandwich at retail outlets. Journal of Milk and Food Technology 34: 289-293.
  14. Barnet, H.L. and Barry, B.H. (1972). Illustrated genera of imperfect fungi. 3rd Ed. Burgess Publication Co., Minneapolis. pp. 63 -70.
  15. Beuchat, L, R. and Ryu, J.H. (2004). Produce handling and processing practices, Emerging Infectious Diseases, 3:459-465.
  16. Brayan, F.L. (1973). Activities of the Centre for Disease Control in public health problems related to the consumption of fish and fishery products. In: C.O. Chichester & H.O. Graham (Eds.). Microbiology Safety for Fishery Products. Academic Press Inc. New York.
  17. British Medical Journal (BMJ). (1990). Food handlers and food poisoning. 300, 208.
  18. Bryant, R.G. (2007). Selective enterotoxin production by a Staphylococcus aureus strain implicated in foodborne outbreak. J. Food Protect, 151:130-131.
  19. Cappuccino, J. and Macfaddin, J.F. (2005). Biochemical tests for the identification of medical bacteria. 2nd Edn.
  20. Centers for Disease Control and Prevention (2012). Epidemiology Program Office, Division of Public Health Surveillance and Informatics, Nationally Notifiable Infectious Diseases, United States. http://www.cdc.gov/osels/ph_surveillance/nndss/phs/infdis.htm (7/12).
  21. Centers for Disease Control and Prevention. Cholera and Other Vibrio Illness Surveillance System. http://www.cdc.gov/nationalsurveillance/cholera_vibrio_surveillance.html. (7/12).
  22. Centers for Disease Control and Prevention (2017). Cholera in Africa. Available online: https://www.cdc.gov/cholera/africa/index.html.
  23. Collier, L., A., Balows, and Sussman, M. (1998). Microbiology and Microbial Infections, 9th ed, vol. 4. Arnold, London, Sydney, Auckland, New York.
  24. Cowan, S.T. (1985). Cowan and Steel’s Manual for the Identification of Medical Bacterial (3rd Edn). Cambridge University Press, London, pp. 81-100.
  25. Cowan, S.T. (1985). Cowan and Steel Manual for the Identification of Medical Bacteria. Cambridge University Press, London. pp.231.
  26. Dambo, W, B. (1993). Tolerance of the periwinkle pachymalania auria and Tympatonus fuscatus to Redifined oils Environmental pollution. 79 (3): page 293-296.
  27. Deekae, S. N. and Idoniboye-Obu, T.I.E. (1995). Ecology and chemical composition of commercially important molluscs and crabs of the Niger Delta, Nigeria. Environment and ecology. Kalyani. 13 (10: 136 – 142.
  28. Ekanem, E.O. and Otti, B.N. (1997). Total plate count and coliform levels in Nigeria periwinkle in fresh and brackish water, Food control, (8): page 87-89.
Read More

Author Biography

Obhioze Augustine Akpoka, Igbinedion University
Faculty and Researcher
Download this PDF file
PDF
Statistic
Read Counter : 344 Download : 529

Downloads

Download data is not yet available.