Issue

Vol. 4 No. (2) (2020): International Journal of Applied Biology

Date Log

Submitted
Jun 29, 2020
Published
Jul 6, 2021

Occurrence of E. coli O157 H7 from meat products sold in Obinze abattoir, IMO State, Nigeria

https://doi.org/10.20956/ijab.v4i(2).10555
Osazee Ekundayo Izevbuwa
Igbinedion university okada
http://orcid.org/0000-0002-2122-6995
Shadrach Osalumhense Okhuebor
University of Benin, Benin City, Edo State, Nigeria
http://orcid.org/0000-0003-2331-1525

Corresponding Author(s) : Osazee Ekundayo Izevbuwa

osazee.izevbuwa@iuokada.edu.ng

International Journal of Applied Biology, Vol. 4 No. (2) (2020): International Journal of Applied Biology

Abstract

AbstractThis survey was carried out to investigate the occurrence of Escherichia coli O157:H7 in meat products from Obinze abattoir, Imo state, South-Eastern Nigeria. A total of 10 beef samples, 10 processing water samples, 10 table swabs and 20 entrails samples were collected. Pour plate technique was used after a tenfold serial dilution to inoculate on Eosin Methylene Blue (EMB) agar and the E. coli isolates were cultured on Sorbitol MacConkey agar and incubated for 24 hours at 37oC. The isolates were subjected to biochemical tests for identification before antibiotic sensitivity test was carried out using the disc diffusion (Kirby-Bauer) method. From the survey, the entrails had the highest rate of isolation (4.16 ±0.56 log10 Cfu/ml) followed by the beef samples with (3.58 ± 0.01 log10 Cfu/ml). The processing water and the table swabs yielded no growth of E. coli O157:H7. The percentage occurrence of E. coli O157:H7 was also determined and the entrails samples had the highest with (17, 89.5%) followed by the beef samples with (2, 10.5%). The processing water and table swabs samples did not yield growth of E. coli O157:H7 and their percentages were 0. The data obtained were subjected to statistical analysis using the single sample T test which showed that there was no significant difference (p > 0.05) in the rate of isolation of E. coli O157:H7 from the samples. The E. coli O157:H7 isolated showed 100% sensitivity to all the antibiotics used. The presence of E. coli O157:H7 implies that these food samples from the Obinze abattoir, if consumed could be a potential public health hazard to the community. Therefore, strict adherence to quality control measures should be implored in order to reduce contamination and food borne illnesses.Keywords: Escherichia coli O157:H7, public health, hazard, contamination, occurrence
References
  1. Arnold, K.W. and Kaspar, C.W. (1995). Starvation and stationary phase induced acid tolerance in Escherichia coli O157:H7. Applied and Environmental Microbiology, 61:2037-2039.
  2. Bavaro, M.F. (2012) E. coli O157:H7 and other toxigenic strains: the curse of global food distribution. Current Fungal Infection Reports, 14(4):317-323.
  3. Chigor, V.N., Umoh, V.J., Smith, S.I., Igbinosa, E.O. and Okoh, A.I. (2010). Multidrug resistance and plasmid patterns of Escherichia coli O157 and other E. coli isolated from diarrhoeal stools and surface waters from some selected sources in Zaria, Nigeria. Intentional Journal of Environmental Public Health, 7:3831-3841.
  4. Coia, J.E. (1998). Clinical, microbiological and epidemiological aspects of Escherichia coli O157:H7 infection. FEMS Immunology and Medical Microbiology, 20:1-9.
  5. Dahiru, M., Uraih, N., Enabulele, S.A. and Shamsudeen, U. (2008). Prevalence of Escherichia coli 0157:H7 in fresh and roasted beef in Kano City, Nigeria. Bayero Journal of Pure and Applied Sciences, 1(1):39-42.
  6. Enabulele, S.A. and Uraih, N. (2009). Enterohaemorrhagic Escherichia coli 0157:H7 Prevalence in meat and vegetables sold in Benin City, Nigeria. African Journal of Microbiology, 3(5): 276-279. Available at: http://www.academicjournals.org/ajmr
  7. Elder, R.O., Keen, J.E., Siragusa, G.R., Barkocy-Gallagher, G.A., Koohmaraie, M. and Laegreid, W.W. (2000). Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proceeding of the National Academy Sciences, 97:2999-3003.
  8. Farougou, S., Sessou, P., Yehouenou, B. and Dossa, F. (2012). Microbiological quality of raw milk processed from cows raised under extensive system in the republic of Benin. Research Journal of Microbiology, 7(7):337-343.
  9. Food and Agricultural Organization (FAO), (1979). Mannuals of Food quality Control for Microbiological Analysis.
  10. Hancock, D.D., Besser, T.E., Kinsel, M.L., Tarr, P.I., Rice, D.H., and Paros, M.G. (1994). The prevalence of Escherichia coli O157.H7 in dairy and beef cattle in Washington State. Epidemiology and Infection, 113:199-207.
  11. Hancock, D.D., Besser, T.E. and Rice, D.G. (1998). Multiple source of Escherichia coli 0157 in feed lots and diary farms in the northrestorn USA. Preventive Veterinary Medicine, 35:11-19.
  12. Itelima, J.U. and Agina, S.E. (2011). The occurrence of Escherichia coli O157:H7 in market and abattoir meat in Plateau State, Nigeria. Global Journal of Environmental Sciences, 10(1&2):47-55.
  13. Karch, H., Janetzki-Mittmann, C., Aleksic, S. and Datz, M. (1996). Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture. Journal of Clinical Microbiology, 34:516-519.
  14. Mead, P.S., Slutsker, L., Dietz. V., McCaig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M. and Tauxe, R.V. (1999). Food related illness and death in the United States. Journal of Emerging of Infectious Disease, 5(5): 607-25.
  15. Nataro, J.P. and Kaper, J.B. (1998). Diarrheagenic Escherichia coli, Clinical Microbiology Review, 11:142.
  16. Nkanga, E.J. and Uraih, N. (1981). Prevalence of Staphylococcus aureus in meat samples from traditional market in Benin City Nigerian and possible control by use of condiments Journal of food protection, 44:4-8.
  17. Okeke, I.N., Fayinka, S.T. and Laminkaran, A. (2000). Antibiotic resistance in Escherichia coli from Nigerian students, 1986-1998. Emerging Infectious Disease, 6:393-396.
  18. Okolocha, E.C., Kwaga, J.K.P., Umoh, J.U. and Ajogi, I. (2006). Risk Assessment of Pathogenic Escherichia coli in Ready to Consume Food of Animal origin (Doctoral Dissertation, Ahmadu Bello University, Zaria). 86-87
  19. Palumbo, S.A., Call, J.E. Schultz, F.J. and Williams, A.C. (1995). Minimum and maximum temperatures for growth and verotoxin production by hemorrhagic strains of Escherichia coli. Journal of Food Protection, 58:352-356.
  20. Paton, J.C. and Paton, A.W. (1998). Pathogenesis and Diagnosis of Shigatoxin Producing Escherichia coli Infections. Clinical Microbiology Reviews, 11:450-479.
  21. Riley, L.W., Remis, R.S. Helgerson, S.D. McGee, H.B. Wells, J.G. Davis, B.R. Hebert, R.J. Olcott, H.M. Johnson, L.M. Margrett, N.T. Blake, P.A. and Cohen, M.L. (1983). Hemorrhagic colitis associated with a rare Escherichia coli serotype. The New England Journal of Medicine, 308:681-685. Available at: http://meridian.allenpress.com/doi/pdf/10.4315/0362-028X-64.6.862
  22. Salome, Y.T., Jacob, K.P.K., Mohammed, B., Junaidu, K., Veronica, J.U., Sabo, E.Y. and Andrew, J.N. (2014). Occurrence of Escherichia coli O157 in Retailed-Beef and Related Meat Products in Zaria, Nigeria. Food and Nutrition Sciences, 5:481-487.
  23. Schlundt, J. (2001). Emerging food borne pathogens. Biomedical Journal of Environmental Science, 14(1-2):44-52.
  24. Uhitil, S., Jaksïc S., Petrak, T. and Botka-petrak, K. (2001). Presence of Escherichia coli O157:H7 in Ground Beef and Ground Baby Beef Meat. Journal of Food Protection, 64(6):862-864.
  25. Walsh, C., Duffy, G., O`Mahony, R., Fanming, S., Blair, I.S. and McDowell, D.A. (2006) Antibacterial resistance in irish isolates of Verotoxigenic Escherichia coli– VTEC. International Journal of Microbiology, 109:173-178.
  26. Wells, J. E., Barry, E.D. and Varel, V.H. (2005) Effects of common forage phenolic acids on Escherichia coli 0157:H7 viability in bovine faeces. Journal of Applied and Environmental Microbiology, 71(12):7974-7979.
  27. WHO (World Health Organization). 1997. Prevention and Control of Enterohaemorrhagic Escherichia coli (EHEC) Infections (WHO/FSF/FOS/97.6). Food Safety Unit, Programme of Food Safety and Food Aid, World Health Organization, Geneva.
  28. Zhao, T., Doyle, M.P., Share, J., Garter, L., (1995). Prevalence of E. coli O157:H7 in a survey of dairy herd. Applied and Environmental Microbiology, 61:1290-1293.
Read More

Author Biographies

Osazee Ekundayo Izevbuwa, Igbinedion university okada
Department of biological sciences (microbiology)  assistant lecturer
Shadrach Osalumhense Okhuebor, University of Benin, Benin City, Edo State, Nigeria
Department of microbiology, faculty of science (researcher)
Download this PDF file
PDF
Statistic
Read Counter : 495 Download : 586

Downloads

Download data is not yet available.