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Submitted
May 30, 2019
Accepted
May 30, 2019
Published
May 30, 2019
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Abstract

Xylanases is an extracellular enzyme that has prospects as enzymes that hydrolyze hemicellulose (xylan). In this study, carried out isolatied of bacteria from the hot springs Makula', Tana Toraja and determine the optimum conditions in producing the xylanase enzyme. The steps being taken are the rejuvenation of bacteria, the manufacture medium inoculum and the production medium, the measurement OD (Optical Density) measurements of protein and testing activities xylanase. The results obtained showed that the production time for B. stearothermophilus SL3A is in the 48 hours with a value of 0.1237 activity mU/mL and B. stearothermophilus SL3S at the 60 hours with a value of 0.1593 activity mU/mL. B. stearothermophilus SL3A have a protein content of 9.828 mg/mL and for B. stearothermophilus SL3S have a protein content of 10.07 mg/mL. Characteristics bacterial xylanase from B. stearothermophilus SL3A and B. stearothermophilus SL3S work optimally at pH 7 a temperature of 45 ºC. Crude extract of the isolated xylanase enzyme can hydrolyze substrate is xylan corn cobs. The addition of metal CaCl2, MgCl2, CaCl2 can increase the activities of the enzyme xylanase and CoCl2 decreases the activities of enzymes that are inhibitors.

Keywords

Bacillus stearothermophilus xylan xylanases

Article Details

How to Cite
Denta Putri, L., Natsir, H., & Dali, S. (2019). Thermophilic Xylanase Production Rom Isolates of Macula’ Hot Springs Bacteria Using Corn Cobs Waste Media. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 10(2), 25-41. https://doi.org/10.20956/ica.v10i2.6652
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References

  1. Budiman, A., and Setyawan, S., 2013, Effect of Substrate Concentration, Incubation Length and Ph in the Process of Isolating Xylanase Enzymes Using Rice Straw Media, Chemical Engineering Department, Faculty of Engineering, Diponegoro University.
  2. Cappucino, J.G., 1983, Microbiology: A Laboratory Manual, Addison Wesley, Publishing Company.
  3. Djide, M.N., and Sartini, 2006, Basic Pharmaceutical Microbiology, Hasanuddin University, Makassar.
  4. Habibie, F.M., Wardani, A.K., and Nurcholis, M., 2014, Isolation and Identification of Molecular Xylanase-Producing Thermophilic Microorganisms from Lapindo Hot Mud, Food Journal and Agroindustry, 2 (4): 231-238.
  5. Lay, W., 1994, Microbial Analysis in Laboratories, PT. Raja Grafindo Persada, Jakarta.
  6. Nakamura, S., Wakabayashi, K., Nakai, R., Aono, R., and Horikoshi, K., 1993, Purification and Some Properties of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strains 41M-1, Applied and Environmental Microbiology, 59 (7): 2311-2316.
  7. Natsir, H., Patong, A.R., Suhartono, M.T. and Ahmad A., 2010, Production and Characterization of Chitinase Enzymes from Hot Spring in South Sulawesi, Bacillus sp. HSA3-1a, Indo. Journal. of Chem, 10 (2): 256–260.
  8. Natsir, H., Patong, A.R., Suhartono, M.T. and Ahmad, A. 2013. Isolation and Purification of Thermostable Chitinase Bacillus licheniformis Strain HSA3-1a From Sulili Hot Springs in South Sulawesi, Indonesia, Int. Journal of Pharm. Bio Sciences, 4 (3): 1252–1259.
  9. Palaniswamy, M., Arulanandham, TV, and Angayaranni, J., 2012, Production of Xylanase by Litter Degrading Fungal Species Using Agro-Industrial Wastes as Substrates by Solid State Fermentation, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3 (1 ): 143-149.
  10. Patong, A.R., 2013, Food Chemistry Analysis, Dua Satu Press, Makassar.
  11. Poedjadi, A., 2012, Basics of Biochemistry, UI-Press, Jakarta.
  12. Prayitno, D.A., and Rachmawaty, R., 2011, Use of Enzymes in the Food Industry, Paper Enzyme Technology, Chemical Engineering FT Diponegoro University, Semarang.
  13. Prima, R.E., 2012, Production and Characterization of Xylanase Coarse Extract from Acinetobacter baumanii M-13.2A, Thesis, published, Faculty of Mathematics and Natural Sciences, Department of Biology, University of Indonesia.
  14. Richana, N., 2002, Production and Prospects of Xylanase Enzymes in Bioindustry Development in Indonesia, Agro Bio Bulletin, 5 (1): 29-36.
  15. Richana, N., and Lestina, P., 2003, Xylanase Production for Bioconversion of Soybean Seed Waste, Center for Biotechnology Research and Agricultural Genetic Resources.
  16. Richana, N., Irawadi T.T, Nur M.A., Syamsu K., and Arkenan, Y., 2007, Xilan Extraction from Corn Cobs, Postharvest Journal, 4 (1): 38-43.
  17. Richana, N., Irawadi T.T, Nur M.A., and Syamsu K., 2008, Isolation of Identification of Xylanase Producing Bacteria and Characterization of Enzymes, Journal of Agro Biogen, 4 (1): 24-34.
  18. Septiningrum, K., and Apriana, C., 2011, Xylanase Production from Corn Cob with Bioprocess System Using Bacillus circulans for Pre-Bleaching Pulp, Journal of Industrial Research, 5 (1): 87-97.
  19. Setyawati, I., 2006, Production and Characterization of Microbial Xylanase Isolated from Corn Cobs, Thesis, unpublished, Faculty of Agricultural Technology, Bogor Agricultural University.
  20. Sopandi, T., and Wardah, 2013, Food Microbiology, Andi Yogyakarta, Yogyakarta.
  21. Susilowati, PE, Raharjo S., Kurniawati D., Rahim R., Sumarlin, and Ardiansyah, 2012, Xylanase Production from Sonai Hot Springs Isolate, Southeast Sulawesi, using Agricultural Waste, Indonesian Naturals Journal, 14 (3): 199- 204.
  22. Thoyib, H., Setyaningsih R., and Suranto, 2007, Selection and Identification of Xylanase-producing Alkaliphilic Bacteria from Bukit Krakitan, Biotechnology, 4 (1): 6-12.
  23. Trismilah and Waltam D.S., 2009, Xylanase Production Using Media for Agriculture and Plantation Waste, Journal of Environmental Technology, 10 (2): 137-144.