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Abstract

This research aimed to know the activity of bioactive protein, extracted from Hydroid (Aglaophenia cuppressina), in inhibiting the growth of pathogenic bacteria. Samples were taken from Lae-lae Island in South Sulawesi. Methods used were the Lowry method for determining the protein concentration and the agar diffusion method  for testing the antibacterial activity. Extraction of Hydroid was conducted by making use of buffer solution, (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl, 1% β-mercaptoethanol, and 0.5% Triton X-100). Protein was purified by ammonium sulfate precipitation at 20%, 40%, 60% and 80% saturation followed by dialysis. Results showed that the protein fraction of Aglaophenia cuppressina before dialysis with 40 % ammonium sulfate saturation had the highest antibacterial activity to Staphylococcus aureus and Escherichia coli with inhibition zones of  14.58 and 18.05 mm, respectively. In addition, the protein fraction of Aglaophenia cuppressina after dialysis with 40 % saturation also had the highest antibacterial activity to the tested bacteria with inhibition zones of  13.95 and 11.78 mm, respectively. The minimum inhibition concentration to the tested bacteria and Escherichia coli at 40% ammonium sulfate (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl 2 saturation was 6000 µg/mL with inhibition zones of 9.78 and 8.73 mm, respectively. Based on the bioactivity test of the Hydroid protein fraction using the agar diffusion method, it can be concluded that the activity characteristic of the fraction is bacteriostatic.Key words: Bioactivity, hydroid, bioactive protein, antibacterial, inhibition zone.

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How to Cite
Umriani, N., Rauf Patong, A., & Ahmad, A. (2016). Studi Bioaktivitas Antibakteri Fraksi Protein Hidroid Aglaophenia cupressina Dari Pulau Lae-Lae Sulawesi Selatan. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 1(1), 6-14. https://doi.org/10.20956/ica.v1i1.2447
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References

  1. Brusea, R., Brusea, G.J., 1990, Invertebrates, Sinauer Associates Inc, Massachussets.
  2. Cappucino, 1987, Microbiology:A Laboratory Manual, The Benjamin Cummings Publishing Company Inc, California.
  3. Dahuri, Rokhmin. 2003, Keanekaragaman Hayati Laut, PT Gramedi Pustaka Utama, Jakarta.
  4. Djide, M.N., 2003, Mikrobiologi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin. Makassar.
  5. Doerge, R. F., 1982, Wilson and Gisvold’s Textbook of Organic Medicinal and Pharmaceutical Chemistry, J.B. Lippincott Company, Pensilvania.
  6. Ganiswarna, S. 1995, Farmakologi dan terapi, Edisi IV, Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia, Jakarta.
  7. Hansson, Hans G. 1998, South Scandinavian Marine Cnidaria, (On line), (http: www//.tmbl.gu.se.,diakses 19 Mei 2007).
  8. Huang. L, 1999, Protein dalam Air Mata Obat untuk AIDS?, (On line),(http://www.rad.net.id/warta/wao4701.htm, diakses 12 Juli 2005).
  9. Irianto, K. 2002, Mikrobiologi Menguak Dunia Mikroorganisme, Rama Widya, Bandung.
  10. Jasin, M. 1992, Zoologi Invertebrata, Penerbit Sinar Wijaya, Surabaya.
  11. Jawetz, E., Melnick, J.L. dan Adelberg, E.A. 1984, Mikrobiologi Untuk profesi Kesehatan, Terjemahan oleh H. Tonang, 1992, Jakarta: EGC Penerbit Buku Kedokteran.
  12. Katzung, BG. 1999, Farmakologi Dasar dan Klinik, Penerbit Salemba Medika, Surabaya.
  13. Kozloff, Eugene N. 1990, Invertebrates. Saunders College Publishing, United States.
  14. Krieg, N. R., 1984, Bergey’s Manual Systematic Bacteriology Vol.1, Baltimore, London.
  15. Kusen, J.D., 1993, Dinamika KeanekaragamanTerumbu Karang dan Peranannya Terhadap Perkembangan Wilayah Pesisir,Prosiding Lokakarya Nasional Penyusunan Program Penelitian Biologi Kelautan dan Proses Dinamika Pesisir, LIPI, Jakarta.
  16. Maskoeri, J. 1992, Zoologi Invertebrata, Penerbit Sianar Wijaya, Surabaya.
  17. Mahon, C. R., G. Manuselis., 1995, Textbook of Diagnostic Microbiology, W. B. Saunders Company, Tokyo.
  18. Majalah Poultry Indonesia. 2 Agustus, 2003, Ada Protein Antimikroba Dalam Sekantung Susu.
  19. McConnaughey, Bayard H. 1987, Introduction To Marine Biology, The C. V. Mosby Company, Saint Louis.
  20. Montgomery, R., Conway, T.W. dan Spector, A.A. 1992, Biokimia Berorientasi pada Kasus Klinik, Terjemahan oleh Staf Pengajar bagian Biokimia Fakultas Kedokteran Universitas Indonesia. 1993, Jakarta: Binarupa Aksara.
  21. Naim, R. 2003, Ada Protein Antimikroba Dalam Sekantong Susu, (On line), (http://www.poultryindonesia.com/modules.php?name=News&file=article&sid=237, diakses 9 Juli 2005).
  22. Pelczar, M.J. dan Chan, E.C.S. 1988, Dasar-Dasar Mikrobiologi 2, Terjemahan oleh Ratna Siri, Teja Imas, S.Sutarmi, Sri Lestari Langka. Jakarta: penerbit Universitas Indonesia.
  23. Page, D.S. 1985, Prinsip-prinsip Biokimia. Edisi kedua. Jakarta: Erlangga.
  24. Rismana,E. 2001, Biota Laut Berpotensi Besar jadi Sumber Bahan Baku Obat dari Industri Farmasi, (Online),(file://A:Biota%20Laut%20Berpotensi%20Besar.htm, diakses 25 Januari 2002).
  25. Ruppert, E.E., Barnes, R.D. 1993, Invertebrata Zoolgy, Sixth Edition. Saunders College Publishing, United States.
  26. Setiono, P. 2002., Infeksi Bakteri Intraseluler Pada Anak. (On line), http://www.Pediatrik.com/kanalpkb34/01InfeksiBakteriIntraselulerParwatiSB.pdf, diakses 25 oktober 2005).
  27. Suparno, 2005, Kajian Bioaktif Spons Laut(Porifera: Demospongiae) Suatu Peluang Alternatif Pemanfaatan Ekosistem Karang Indonesia dalam Bidang Farmasi, (On line), http://72.14.203.104/search?q=cache:uflSEp5vNhoJ:tumoutou.net/pps702_10245/suparno, diakses 9 September 2005).
  28. Setyaningsih. 2004, Resistensi bakteri dan Antibiotik Alami dari Laut, (Online),(http://72.14.203.104/search?q=cache7y-24YyXJEJ:tumoutou.net/pps702_9145/Iriani_sety, diakses 9 September 2005).
  29. Wattimena, J.R. 1991, Farmakodinamika dan terapan Antibiotik. Gadjah Mada University Press. Yogyakarta.
  30. Wheeler, F.M., Volk, A.W., 1988, Mikrobiologi Dasar Jilid I, Erlangga. Jakarta.
  31. Wiryowidagdo, S. 2007, Potensi Obat Dari Laut Belum Dimaksimalkan,(Online),(http://www.bppt.go.id/index.php?option=com_content&task=view&id=1548&Itemid=30, diakses 25 Mei 2007).