Anti-free radical activity test of endophytic fungal fermentate extract on the Snake Plants (Sansevieria trifasciata Hort. Ex Prain) using the TLC-Autography method

Abstract

Degenerative diseases occur due to damage to cells, fat tissue, and the immune system caused by several factors. One theory that causes degenerative diseases is the presence of free radicals. The Snake plant is one of the plants in Indonesia that has long been known as a plant that is useful as anti-free radicals. The research was conducted by isolating and purifying endophytic fungi and determining the autogram profile of free antiradical activity from The Snake plant. From the purification results obtained 7 isolates, followed by macroscopic examination and fermentation of each isolate. The results of the screening test showed that 2 fermentates were active as anti-free radicals, namely AF 1 and AF 3. Fermentate isolates AF 1 and AF 3 were identified by TLC-autography using a mixture of n-hexane: ethyl acetate (7:1). TLC-autography test results by spraying DPPH isolates AF 1 and isolates AF 3 showed yellow spots on a purple background with Rf1 values ​​of 0.90 and Rf2 of 0.2 in AF1 isolates and Rf1 values ​​of 0.83 and Rf2 of 0.16 on AF3 isolates. potential as anti-free radicals. From the results of this study, it was concluded that there was anti-free radical activity in the Snake plant.