Article Sidebar

Submitted
Jul 20, 2020
Accepted
Dec 18, 2020
Published
Dec 19, 2020
Download
PDF
Statistic
Read Counter : 305 Download : 320

Downloads

Download data is not yet available.

Main Article Content

Abstract

Elephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.lephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.

Keywords

Antibacterial endophytic fungi elephant ginger TLC-Bioautography bacteria that cause skin infections.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author Biography

seniwati seniwati, Universitas Hasanuddin

Department of Chemistry
How to Cite
seniwati, seniwati, Aprilia, L., Rusli, R., & Naid, T. (2020). Antibacterial Activity Test of Elephant ginger (Zingiber officinale Rosc.) Endophytic Fungi Variation of Elephants Against Bacteria That Cause Skin Infections. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 13(2), 68-72. https://doi.org/10.20956/ica.v13i2.10771
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Adriani (2015) ‘Aktivitas Antibakterial Fungi Endofit Caulerpa racemosa Terhadap Bakteri Escherichia coli dan Staphylococcus aureus’, Prosiding seminar Nasional Mikrobiologi Kesehatan dan Lingkungan, (2014), pp. 11–15.
  2. Arifin, Z. (2012) AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL JAHE MERAH (Zingiber officinale Roscoe var rubrum) TERHADAP Staphylococcus aureus, Escherichia coli, DAN Candida albicans, Fakultas Farmasi, Universitas Muhammadiyah Semarang.
  3. Dianasari, D. et al. (2020) ‘Potensi Ekstrak Etanol dan Fraksi-Fraksinya dari Tiga Varietas Jahe sebagai Agen Antibakteri terhadap Staphylococcus aureus Potency of Ethanolic Extracts and Its Fractions of Three Ginger Varieties as Antibacterial Agent Against Staphylococcus aureus Bakteri penyebab Bahan rimpangkjaheegajah ( Zingiber officinale var . Tanaman Politeknik Negeri Jember ). Bahan- Pembuatan EkstraklEtanol Rimpang Jahe dimaserasi’, 17(1), pp. 9–16.
  4. Gazali, A. M. F., Anam, S. and Khumaidi, A. (2016) ‘Isolasi Senyawa Antibakteri Ekstrak Etanol Akar Krokot ( Portulaca oleracea Linn ) Menggunakan Bakteri Uji Staphylococcus aureus Isolation of Antibacterial Ethanolic Extract Compound from Purslane ( Portulaca oleracea Linn ) Roots Using Bacteria Test of S’, Jurnal of Nature Science, 5(1), pp. 49–59.
  5. Gouda, S. et al. (2016) ‘Endophytes: A treasure house of bioactive compounds of medicinal importance’, Frontiers in Microbiology, 7(SEP), pp. 1–8. doi: 10.3389/fmicb.2016.01538.
  6. Hamzah, T. N. T. et al. (2018) ‘Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, Rhizophora mucronata, and identification of potential antagonists against the soil-borne fungus, Fusarium solani’, Frontiers in Microbiology, 9(JUL), pp. 1–17. doi: 10.3389/fmicb.2018.01707.
  7. Kaitu, R. A. M., Boy Rahardjo Sidharta and Atmodjo, K. (2013) ‘Aktivitas antibakteri fungi endofit jahe merah (’, Jurnal Biologi, pp. 1–15.
  8. Khusnul, Wahyuni, H. S. and Virgianti, D. P. (2015) ‘IDENTIFIKASI JAMUR ENDOFIT PADA DAUN CINCAU (Cyclea barbata Miers) dan UJI ANTAGONIS TERHADAP Salmonella typhi’, Jurnal Kesehatan Bakti Tunas Husada, 14, pp. 1–7.
  9. Mani, M. M. et al. (2014) ‘Antimicrobial activity and phytochemical screening of various parts of Ixora coccinea’, Journal of Medicinal Plants Research, 8(10), pp. 423–429. doi: 10.5897/jmpr11.1281.
  10. Mishra, A. K., Yadav, P. and Mishra, A. (2016) ‘A Systemic Review on Staphylococcal Scalded Skin Syndrome (SSSS): A Rare and Critical Disease of Neonates’, The Open Microbiology Journal, 10(1), pp. 150–159. doi: 10.2174/1874285801610010150.
  11. Noverita, Fitria, D. and Sinaga, E. (2009) ‘Jamur Endofit dari Daun dan Rimpang’, Jurnal Farmasi Indonesia, 4(4), pp. 171–176.
  12. Pratiwi, R. E. (2016) Uji aktivitas antibakteri fermentat isolat fungi pada ampas sagu asal kota palopo secara KLT-Bioautograf. Universitas Muslim Indonesia.
  13. Reckow, V., Widayat, W., Rijai, W. (2016) ‘Pendahuluan Bawang dayak ( Eleutherine palmifolia ( L .) Merr .)’, Laboratorium Penelitian dan Pengembangan ‘FARMAKA TROPIS’ Fakultas Farmasi Universitas Mulawarman, Samarinda, Kalimantan Timur, pp. 20–21.
  14. Sari, Kartika Indah Permata, Periadnadi, Nasir, N. (2013) ‘Uji Antimikroba Ekstrak Segar Jahe-Jahean ( Zingiberaceae ) Terhadap Staphylococcus aureus , Escherichia coli dan Candida albicans Antimicrobial test of ginger fresh extract ( Zingiberaceae ) against Staphylococcus aureus , Escherichia coli and Candida al’, Jurnal Biologi Universitas Andalas, 2(1), pp. 20–24.
  15. Widowati, T. et al. (2016) ‘Isolasi dan Identifikasi Kapang Endofit dari Tanaman Kunyit (curcuma longa l.) Sebagai Penghasil Antioksidan’, Biopropal Industri, 7(1), pp. 9–16.
  16. Yunus, M. I. (2015) Isolasi fungi endofit dari daun Jatropa multifida L sebagai Antibiotika dan Antiradikal bebas. Universitas Muslim Indonesia.