Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula
Abstract
Breast milk is the best source of nutrition because it contains a variety of healthy compounds and immunity. Several studies of gut microbiota has proven its important role in the development of the immune system. This study aims to characterize the gut microbiota of babies born normally with breastfeeding and formula milk. This research using cross sectional study design with purposive sampling as sampling technique. Samples used were babies born normally as many as 30 infants that divided into two groups namely infants who given breast milk and combinated breastfeeding and formulamilk of 15 infants each. Stool samples were taken by swab technique, the DNA was extracted using a DNA kit continued DNA amplification by PCR with 16SrRNA universal primers then DNA sequencing. Data from DNA sequencer analized using a software of MEGA5 and BLAST attained on the NCBI link. The result showed that the group of infants who were breastfed plus formula milk had more varied intestinal microbiota than the group of infants who were only given breast milk. It can be concluded that intestinal microbiota that dominates the intestines of infants who are given breast milk can be a probiotic in the growth of the baby and the development of the immune system.
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2. Dinas Kesehatan Provinsi Sulawesi Selatan. Rencana Strategi Bidang Kesehatan 2017. Makassar: Dinas Kesehatan Sulawesi Selatan; 2017.
3. Erniawati dan Kamaruddin M. Asuhan Kebidanan III (Nifas). Bandung: Manggu Makmur Tanjung Lestari; 2019.
4. Devriany A, Wardani Z, danYunihar. Perbedaan Status Pemberian ASI Eksklusif dan Non-Eksklusif terhadap Perubahan Panjang Badan Bayi Neonatus. Media Kesehatan Masyarakat Indonesia. 2018;14(1):44-51.
5. Gritz EC, Bhandari V. The Human Neonatal Gut Microbiome: a Brief Review. Frontiers in Pediatrics. 2015;3(17):1-12.
6. Astuti S. Asuhan Kebidanan Nifas dan Menyusui. Jakarta: Erlangga; 2015.
7. Dietert RR, Dietert JM. The Microbiome and Sustainable Healthcare. Healthcare. 2015;3(1):100-129.
8. Frank NM, Lynch KF, Ulla U, Jimin Y, et al. The Relationship Between Breastfeeding and Reported Respiratory and Gastrointestinal Infection Rates in Young Children. BMC Pediatrics. 2019;19(339):1-12.
9. Massi N, Ahmad A, Rafiah S, Kamaruddin M, Hidayah N, Handayani I, Halik H and Abidin RS. Sputum Microbiota in Normal Human, New Tuberculosis, and Recurrent Tuberculosis Patients as Identified by 16SrRNA Sequencing. Journal of Genetic Engineering and Biotechnology. 2019.
10. Kamaruddin M, Triananinsi N, Minarti, Sumarni, Nurqalbi dan Massi N. Metagenomic and Development of the Gut Infants in Bulukumba. Proceeding of the 13th Young Scientist Conference, Yamaguchi Shi; 2017.
11. Teguh SH. Profil Mikroba Usus pada Anak Usia 2-12 Tahun dengan Diare dan Non-Diare di Jakarta Utara Indonesia. [Thesis]. Jakarta: Program Pendidikan Dokter Spesialis Mikrobiologi Klinik, Fakultas Kedokteran Universitas Indonesia; 2014.
12. Pratiwi PS. Mikrobioma: Pemahaman Baru Tentang Peran Mikroorganisme dalam Kehidupan Manusia. E-Journal Kedokteran Indonesia. 2016;4(2):71-75.
13. Fitri EBH dan Beivy JK. Interaksi antara Mikrobiota Usus dan Sistem Kekebalan Tubuh Manusia. Jurnal Ilmiah Sains. 2017;17(1): 35-42.
14. Ruairi CR, Amee RM, Finlay BB, dan Andrew JP. The Human Microbiome and Child Growth-First 1000 Days and Beyond. Trends in Microbiology. 2019;27(2):131-147.
15. Nakayama J, Watanabe K dan Lee YK, et al. Diversity in Gut Bacterial Community of School-Age Children in Asia. Scientific Reports. 2015;5(8397):1-11.
16. Sommer F. The Gut Microbiota-Master of Host Development and Physiology. Nature Reviews Microbiology: 2013; 11: 227-238.
17. Amitay EL, and Lital KB. Breastfeeding and Childhood Leukemia Incidence. JAMA Pediatrics. 2015;169(6):77-93.
18. Chris D. Faecal Microbiota Transplantation: a Review of FMT as an Alternative Treatment for Clostridium Difficile Infection.Bioscience Horizons. 2016;9:1-14.
19. Sudarmono PP. Pemahaman Baru tentang Peran Mikroorganisme dalam Kehidupan Manusia. Mikrobioma. 2016:4(2):1-7.
20. Chyn BW dan Jin-Zhong X. Beneficial Effects of Bifidobacterium Longum Subsp. Longum BB536 on Human Health: Modulation of Gut Microbiome as the Principal Action. Journal of Functional Food. 2019;54(3):506-519.
21. Priyantoro dan Sigit T.Gut Microbiota dan Irritable Bowel Disease. Medical Review. 2015.
22. Kumbhare SV, Patangia DV, Patil RH, et al. Factors Influencing the Gut Microbiome in Children: From Infancy to Childhood. Journal of Bioscience. 2019;44(2):49.
23. Yu G, Guoqiang Z, Xiali Q, Shuinin H, Bangnao W and Haiong C. The Potential Role of Gut Mycobiome in Irritable Bowel Syndrome. Frontiers in Microbiology. 2019;10(1894):1-12.
24. Sahoo K, Sahoo B, Choudhury AK, Kumar R, Bhadoria AS. Childhood Obesity: Causes and Consequences. Journal of Family Medicine and Primary Care. 2015;4(2):187-192.
Authors
Kamaruddin, M., Triananinsi, N., Sampara, N., Sumarni, S., Minarti, M., & RA, A. M. (2020). Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula. Media Kesehatan Masyarakat Indonesia, 16(1), 116-126. https://doi.org/10.30597/mkmi.v16i1.9050
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