Efektivitas Ekstrak Kulit Buah Limau Kuit (Citrus Amblycarpa) sebagai Larvasida Aedes Aegypti Instar III
Abstract
Various negative effects from the use of chemical larvacides encourage research about natural larvacides,one of which is extract of lime skin extract. This study aimed to determine the effectiveness of lime skin extractas a natural larvacide against the death of Aedes aegypti larvae. This study uses a true experiment design with apost test only control group design. The object of the research was the lime peel extract. The sample size in thisstudy was 450 instar III larvae with Simple Random Sampling technique and treated with various concentrationsof lime skin extract (2ml/100ml, 3ml/100 ml, 4ml/100ml, and 5ml/100 ml) for 10 hours. Positive controls use abate0.01 gr / 100ml. The results showed that all treatment groups could kill 100% of larvae. At a concentration of 4.0ml / 100ml and 5.0 ml / 100ml experienced 100% mortality after 6 hours of measurement and the positive controlgroup experienced 100% death after 4 hours of measurement. The results of the probit analysis show that the valueof LT50 concentration of 5.0% is 2.58 hours and the value of LT99 is 5.86 hours. The Kruskal Wallis test resultsshowed no significant differences (p>0.05) larvae mean mortality between treatment groups and the control group.Giving skin extracts of lime juice can be a natural larvicidal alternative that can be used by the community.
References
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2. Harapan H, Michie A, Mudatsir M, Sasmono RT, Imrie A. Epidemiology of Dengue Hemorrhagic Fever in Indonesia: Analysis of Five Decades Data From the National Disease Surveillance. BMC Research Notes.2019;12(350):1-6.
3. Angeline G, Kularatnam M, Jasinge E, Gunasena S, Samaranayake D. Evaluation of Biochemical and Haematological Changes in Dengue Fever and Dengue Hemorrhagic Fever in Sri Lankan Children: a Prospective Follow up Study. BMC Pediatrics. 2019;19(87):1-9.
4. Lauer SA, Sakrejda K, Ray EL, Keegan LT, Bi Q, Suangtho P. Prospective Forecasts of Annual Dengue Hemorrhagic Fever Incidence in Thailand, 2010–2014. Proceedings of the National Academy of Sciences.2018;115(10):2175-2182.
5. Yang, Fen Lin, Hui Yang, Lin Zhang, Sen-Hai Fang, Xiao-Fen Zhan L-yY. The Analysis of Clinical and Laboratory Data: a Large Outbreak of Dengue Fever in Chaozhou, Guangdong Province, China. Archives of Virology. 2019;164(8):2131-2135.
6. Ningrum DS, Pramatama S, Wijayanti M. Mosquito Larvacidal Activity of Zingiber Montanum Rhizome Extract Against Aedes Aegypti Larvae. BALABA. 2019;15(1):33-40.
7. WHO. Global Strategy for Dengue Prevention and Control. WHO; 2012.
8. Manyullei S, Ishak H, Ekasari R. Perbandingan Efektivitas Air Perasan Kulit Jeruk Manis dan Temephos terhadap Kematian Larva Aedes Aegypti Comparative Efficiency of the Juice of Sweet Orange Peel and Temephos on Aedes Aegypti Larvae Efficacy. MKMI.2015;11(1):23-31.
9. Nuning Irnawulan Ishak K. The Effect of Climate Factors for Dengue Hemorrhagic Fever in Banjarmasin City, South Kalimantan Province, Indonesia, 2012-2016. Public Health of Indonesia. 2018;4(3):121-128.
10. Ratih Dewi Dwiyanti, Rion Dediq AT. Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp. Medical Laboratory Technology Journal. 2017;3(1):93-97.
11. Muangmoon R, Junkum A, Chaithong U. Natural Larvicides of Botanical Origin Against Dengue Vector Aedes Aegypti (Diptera: Culicidae). Southeast ASIAN J Trop Med Public Health. 2018;49(2):227-239.
12. Azidi Irwan, Kamilia Mustikasari DA. Pemeriksaan Pendahuluan Kimia Daun, Kulit dan Buah Limau Kuit: Jeruk Lokal Kalimantan Selatan. Sains dan Terapan Kimia. 2017;11(2):71-79.
13. Tinneke Lumowa SV PNT. Larvicidal Activity of Syzygium Polyanthum W. Leaf Extract Against Aedes Aegypti L Larvae. Progress in Health Sciences. 2015;5(1):102-106.
14. Lubis R, Ilyas S, Panggabean M. The Effectivity Test of Aloe Vera Leaf Extract to Larvae Aedes SP. Asian Journal of Pharmaceutical Clinical Research. 2018;11(7):262-266.
15. Amalia R. Daya Bunuh Air Perasan Daun Mengkudu (Morinda citrifolia) terhadap Kematian Larva Aedes aegypti. [Skripsi]. Semarang: Universitas egeri Semarang; 2016.
16. Wati WE, Astuti D, Darnoto S. Beberapa Faktor yang Berhubungan dengan Kejadian Demam Berdarah Dengue (DBD) di Kelurahan Ploso Kecamatan Pacitan Tahun 2009. VEKTORA. 2011;3(1):22-34.
17. Husnawati, Prapti Sedijani NL. Efektivitas Ekstrak Etanol Daun Kirinyuh (Eupatorium odoratum L) terhadap Mortalitas Larva Nyamuk Aedes aegypti sebagai Pengayaan Praktikum Fisiologi Hewan. Mataram; 2018.
18. Arimaswati, Sawaluddin OM, Sudrajat HW. Efek Larvasida Ekstrak Biji Buah Pepaya ( Carica papaya L .) terhadap Larva Instar III A edes aegypti L . MEDULA. 2017;4(April):332-343
19. Koneri R, Pontororing HH. Uji Ekstrak Biji Mahoni (Swietenia Macrophylla) terhadap Larva Aedes aegypti Vektor Penyakit Deman Berdarah. MKMI. 2016;12(4):216-223.
20. Yoke Astriani MW. Potensi Tanaman di Indonesia sebagai Larvasida Alami untuk Aedes Aegypti. SPIRAKEL. 2018;8(2017):37-46.
21. Yoma Seivia F. Tarukbua, Edwin De Queljoe WB. Skrining Fitokimia dan Uji Toksisitas Ekstrak Etanol Daun Brotowali (Tinospora Crispa (L.) Hook F . & T) dengan Metode Brine Shrimp Lethality Test (BSLT). PHARMACON Jurnal Ilmu Farmasi. 2018;7(3):330-337.
22. Ifa Ahdiyah KIP. Pengaruh Ekstrak Daun Mangkokan (Nothopanax scutellarium) Sebagai Larvasida Nyamuk Culex sp. Jurnal Sains dan Seni ITS. 2015;4(2):2337-3520.
23. Nur Handayani, Ludfi Santoso, Martini SP. Status Resistensi Larva Aedes Aegypti terhadap Temephos di Wilayah Perimeter dan Buffer Pelabuhan Tanjung Emas Kota Semarang. Jurnal Kesehatan Masyarakat. 2016;4(3):159-166.
24. Indri Ramayanti RF. Uji Efektivitas Larvasida Ekstrak Daun Pepaya (Carica papaya Linn) terhadap Larva Aedes aegypti. Syifa’MEDIKA: Jurnal Kedokteran dan Kesehatan.2016;6(2):79-88.
25. Istiana, Heriyani F, Isnaini. Status Kerentanan Larva Aedes Aegypti terhadap Temefos di Banjarmasin Barat. Jurnal BUSKI: Epidemiologi dan Penyakit Bersumber Binatang (Epidemiology Zoonosis Journal). 2012;4(2):53-58.
Authors
Ishak, N. I., Kasman, K., & Chandra, C. (2019). Efektivitas Ekstrak Kulit Buah Limau Kuit (Citrus Amblycarpa) sebagai Larvasida Aedes Aegypti Instar III. Media Kesehatan Masyarakat Indonesia, 15(3), 302-310. https://doi.org/10.30597/mkmi.v15i3.6533
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